Method for separating and purifying mummified aphids of aphid parasites
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A technology of Aphids cocoon wasps and aphids, applied in the field of biological control, can solve the problems of low product purity, low utilization rate of aphids, and affecting the control effect, so as to improve the quality of aphids, avoid the spread of pathogens, and avoid the emergence of aphids The effect of rate reduction
Active Publication Date: 2021-06-29
云南格瑞生物科技有限公司
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[0007] Aphids are the pupal stage of the wasp, which is a living body in the dormant stage. It is oval in shape, small in size, and the radius is mostly between 0.9-1mm. Most of them are attached to the back of the leaves and near the petioles of the carrier plants. When harvesting, it is easy to cause damage to the cocoon wall of the aphid and affect the emergence of the aphid
Although there are steps of separation and impurity removal in the prior art, the damaged part of the aphid is not treated, resulting in low purity of the product, which in turn affects the control effect, and the utilization rate of the aphid is low
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Embodiment 1
[0033] A method for isolating and purifying Aphids aphids, said method comprising the following steps:
[0034] (1) Preparation of aphid seedlings to be separated: use the flue-cured tobacco variety msk326 as the feeding carrier, adopt the seedling breeding method for feeding, and the sowing density is 576 plants / ㎡; when the feeding carrier grows to 3 leaves and 1 heart, select a single aphid Aphid seedlings with an amount greater than 120 and an actual parasitic rate of 40-60% are used as seed aphids, and the aphids are grafted with the amount of 3 aphids per plant using the grafting method. After the aphids are grafted, ventilation and light transmission management should be strengthened; 12 days after the aphid source, shading management started from 9:00 to 17:00 every day except for cloudy and rainy weather; 24 days after the aphid source was connected, the number of aphids was 37.2 heads / plant, the number of parasitic aphids was 193 heads / plant, and the number of dead aph...
Embodiment 2
[0050] Step (7) Second separation and purification: cut off the leaves of carrier 1, spread them on carrier 2 with the insect-bearing side facing up, until the carrier is fully covered.
[0051] Step (8) Collection of high-purity aphids: after 24 hours, the leaves of the carrier 1 are collected and packaged commercially for the control of aphids;
[0052] All the other are the same as example 1.
example 3
[0054] The variety of flue-cured tobacco is msk326, which is cultivated in a pot until 6 true leaves are used as the carrier 2, and the rest are the same as in Example 2.
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Abstract
The invention discloses a method for separating and purifying mummified aphids of aphid parasites. The method comprises the following steps: (1) preparing mummified aphids to be separated; (2) preparing a carrier 1; (3) performing primary separation and purification; (4) collecting the purified mummified aphids; (5) continuing to culture a material of the carrier 1; (6) preparing a carrier 2; (7) performing secondary separation and purification; (8) collecting the high-purity mummified aphids; and (9) continuing to breed the material of the carrier 2. According to the method, biological characteristics of aphid parasites and aphids are combined, parasitic aphids and aphids are transferred due to plant withering, mummified aphids left on an original carrier can be collected and packaged, and the transferred aphids can be continuously bred. According to the method, material utilization is improved in the production link, the content of impurities and empty shells in the mummified aphid products is reduced, the mummified aphid rate is increased, damage to the mummified aphids is small, transportation and loose keeping of the mummified aphids are facilitated, and large-scale production and application commercialization of aphid parasites are facilitated.
Description
technical field [0001] The invention relates to the technical field of biological control, in particular to a method for separating and purifying the aphid Aphididae. Background technique [0002] Aphids, commonly known as greasy insects or honey insects, belong to the order Hemiptera and can harm wheat, corn, tobacco, cotton, cabbage, radish, soybean, rapeseed, cabbage, peach trees, apples, etc. They not only suck leaf juice, but also spread viral diseases , When the quantity is large, coal pollution is formed, causing a decline in quality. At present, there are more than 4,700 known species in the world, and about 1,100 species are distributed in China. [0003] Aphididae species are endoparasitic wasps of aphids and an important class of natural enemy insects, which have been used to control some important aphids. China is the country with the richest resources of Aphids, the main species are: Aphids, Aphids, A. Cocoon wasp, swallow aphid cocoon wasp, etc. [0004] At...
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