A method for isolation and culture of adult mouse atrial myocytes

Abstract

The invention discloses a method for separating and culturing atrial myocytes of an adult mouse. The method uses two different culture solutions for culturing the atrial myocytes in different culture stages, so as to increase the quantity and quality of the atrial myocytes. During the process, the nutrition and protection of cells are increased, and the damage rate of atrial myocytes is greatly reduced. The application of the plating medium can make the isolated atrial myocytes quickly adapt to the culture environment and improve the efficiency of cell adhesion; the ordinary medium provides a stable temperature, pH and sufficient nutrition for the subsequent culture of atrial myocytes. its good growth. The results show that the method of the present invention can obtain high-yield and high-quality adult mouse atrial myocytes, with low cost, high efficiency and stability, and the isolated atrial myocytes are large in number and high in activity, and can be cultured in vitro for 4-5 days. It provides a good experimental material to study the physiological and pathological functions of adult mouse heart at the cellular level.

Description

technical field [0001] The invention belongs to the technical field of cell separation and culture, in particular to a method for separating and culturing adult mouse atrial myocytes. Background technique [0002] Atrial fibrillation (AF) is a supraventricular arrhythmia characterized by rapid and disordered atrial electrical activity. It is the most common clinical arrhythmia and constitutes one of the major threats to the healthy life of middle-aged and elderly people. Atrial fibrillation has been discovered and studied for more than 100 years, but its mechanism is still not fully understood. [0003] The atrial myocyte model can eliminate the interference of various factors such as hormone levels in the body. It is an indispensable in vitro experimental model for the study of atrial fibrillation. It can be widely used in toxicity experiments, genetic engineering, pathological models, and clinical drug screening. Therefore, in order to achieve the goal of studying the mec...

AI Technical Summary

Problems solved by technology

However, due to the high concentration of (-)-Blebbistatin (25μM) has a strong contraction inhibitory effect on cardiomyocytes, it is extremely difficult to wash it out when changing the medium, which limits the subsequent research on cell function.

In addition, (-)-Blebbistatin is very expensive, which increases the cost of separation and culture technology, and the economy and practicability are very limited

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