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Application of 15 active pharmaceutical ingredients in virus infection resistance

A drug and active ingredient technology, applied in the field of anti-coronavirus infection, can solve problems such as virus mutation, vaccines and antiviral drugs without specific effects for severe pneumonia, and human-to-human infection

Inactive Publication Date: 2021-10-22
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The transmission route of 2019-nCoV virus has not been fully grasped. It is known that it can be transmitted through droplets and contact, and there is human-to-human transmission and medical staff infection. There is a certain risk of community transmission, and the virus may mutate
[0006] Currently, there is no effective vaccine or antiviral drug for severe pneumonia caused by SARS-CoV-2 coronavirus

Method used

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  • Application of 15 active pharmaceutical ingredients in virus infection resistance
  • Application of 15 active pharmaceutical ingredients in virus infection resistance
  • Application of 15 active pharmaceutical ingredients in virus infection resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1. Anti-SARS-CoV pseudovirus invasion activity screening (virus surface spike protein-cell level model)

[0089] Test principle: Huh7 cells are used as virus host cells (susceptible cells), and the test sample blocks the activity of SARS-CoV membrane protein modification of HIV pseudovirus-infected cells, which can reflect the anti-virus of the sample intervening key targets of SARS-CoV infection active. The detection index is the activity level of the reporter gene on the pseudovirus genome.

[0090] testing method:

[0091] Huh7 cells were inoculated in 96-well culture plates one day in advance, and the activity plate and cytotoxicity plate were respectively set up, and placed at 37°C, 5% CO 2 to cultivate. The activity assay plate and the cytotoxicity assay plate were loaded in the same way, and samples of different dilution concentrations and SARS-CoV pseudovirus suspension were added, and a virus control, a cell control, and a sample control were set up....

Embodiment 2

[0099] Example 2. Anti-2019-nCoV pseudovirus invasion activity screening (virus surface spike protein-cell level model)

[0100] Test principle: Huh7 cells are used as virus host cells (susceptible cells), and the test sample blocks the activity of 2019-nCoV membrane protein to modify HIV pseudovirus-infected cells. active. The detection index is the activity level of the reporter gene on the pseudovirus genome.

[0101] testing method:

[0102] Huh7 cells were inoculated in 96-well culture plates one day in advance, and the activity plate and cytotoxicity plate were respectively set up, and cultured at 37°C and 5% CO2. The activity assay plate and the cytotoxicity assay plate were loaded in the same way, and samples of different dilution concentrations and 2019-nCoV pseudovirus suspension were added, and virus controls, cell controls, and sample controls were set. After continuing to culture for 3 days, the cytotoxicity plate was used to measure the cell survival rate by M...

Embodiment 3

[0109] Example 3. Anti-MERS-CoV pseudovirus invasion activity screening (virus-cell level model)

[0110] Test principle: Huh7 cells are used as virus host cells (susceptible cells), and the test sample blocks the activity of MERS-CoV membrane protein to modify HIV pseudovirus-infected cells. This activity can reflect the antiviral effect of the sample on key targets of MERS-CoV infection active. The detection index is the activity level of the reporter gene on the pseudovirus genome.

[0111] testing method:

[0112] Huh7 cells were inoculated in 96-well culture plates one day in advance, and the activity plate and cytotoxicity plate were respectively set up, and cultured at 37°C and 5% CO2. The activity assay plate and the cytotoxicity assay plate are loaded in the same way, and samples of different dilution concentrations and 2019-nCoV pseudovirus suspension are added, and virus controls, cell controls, and sample controls are set. After continuing to culture for 3 days,...

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Abstract

The invention relates to an application of 15 active pharmaceutical ingredients in coronavirus resistance. Specifically, the invention relates to bosutinib, hydroxypiperazine chlorpromazine, iodoamine hydrochloride, flupentixol hydrochloride, nortiline hydrochloride, isobutrazine tartrate, dapoxetine, chlorprothiene hydrochloride, protiline hydrochloride, promethazine hydrochloride, gefitinib, solifenacin succinate and azelastine hydrochloride. The clomastine fumarate and the toremifene or the composition of the clomastine fumarate and the toremifene is used as an inhibitor for inhibiting the activity of coronavirus spike protein; and / or (b) the invention relates to an application of a medicine for treating and / or preventing and relieving related diseases caused by coronavirus infection.

Description

technical field [0001] The present invention relates to the field of medicine, in particular to the application of 15 active ingredients of medicines in antiviral infection, especially the application in anticoronavirus infection. Background technique [0002] Among the acute infectious diseases, most of them are viral infectious diseases, and the incidence rate of viral infectious diseases is high, and the mortality rate is also high. Due to the limited means of detection and diagnosis, outbreaks of new outbreaks caused by new viruses are often characterized by suddenness, randomness, and unpredictability. Health and life safety. [0003] Coronavirus (Coronaviruses) is a single-stranded positive-sense RNA virus, belonging to Nidovirales (Nidovirales) Coronaviridae (Coronaviridae) positive coronavirus subfamily (Orthocoronavirinae), can infect humans, bats, pigs, mice, cattle, horses, goats , monkeys and many other species. There are six known human-infecting coronaviruse...

Claims

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Application Information

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IPC IPC(8): A61K45/06A61K31/5415A61K31/5377A61K31/4725A61K31/496A61K31/135A61K31/138A61K31/382A61K31/55A61K31/343A61K31/137A61K31/40A61P31/14A61P31/16A61P11/00
CPCA61K45/06A61K31/5415A61K31/5377A61K31/4725A61K31/496A61K31/343A61K31/135A61K31/138A61K31/382A61K31/55A61K31/137A61K31/40A61P31/14A61P31/16A61P11/00A61K2300/00
Inventor 左建平唐炜童贤崑杨莉何世君何珮岚周宇
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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