Research method for identifying NK cell depletion in bone marrow of AML patient and application of research method

A NK cell and patient technology, applied in cell-related fields, can solve the problem of not meeting the needs of judging whether AML has relapsed

Pending Publication Date: 2021-10-22
PEOPLES HOSPITAL PEKING UNIV
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Problems solved by technology

However, this finding can no longer meet the needs of current technicians to judge whether AML relapses after HSCT

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  • Research method for identifying NK cell depletion in bone marrow of AML patient and application of research method
  • Research method for identifying NK cell depletion in bone marrow of AML patient and application of research method
  • Research method for identifying NK cell depletion in bone marrow of AML patient and application of research method

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Embodiment Construction

[0025] In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0026] In current technical studies, although T cell depletion has been observed to be associated with a high recurrence rate of AML after HSCT. However, in the current research results, it is not enough to prove whether NK depletion exists in AML, and the exploration of inhibitory receptors and transcription factors is also lacking.

[0027] That is to say, in the prior art, ...

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Abstract

The invention provides a research method for identifying NK cell depletion in bone marrow of an AML patient and application of the research method. The method comprises the following steps: researching the maturity phenotype of NK cells in bone marrow when an AML patient relapses, and after determining the maturity of the NK cells, researching whether an inhibitory receptor is in overexpression on the NK cells when the AML patient relapses based on a receptor marker; and then, further researching the anti-tumor function change and multiplication capacity of the NK cells after recurrence of the AML patient and analyzing transcriptomics to identify the depletion phenomenon of the NK cells in the bone marrow of the AML patient. According to the method provided by the invention, a systematic research method is provided for NK cell depletion caused by leukemia for the first time, the NK cell depletion phenomenon of AML relapse patients after HSCT is proved, and meanwhile, high-frequency expression of NKG2A + NK cells is related to the relapse risk. Therefore, the research method provided by the invention has a huge application value in researching and judging whether the NK cells of the AML patient after HSCT are depleted or not.

Description

technical field [0001] The invention belongs to the technical field of cells, and in particular relates to a research method for identifying the exhaustion of NK cells in the bone marrow of AML patients after transplantation and its application. Background technique [0002] Acute myeloid leukemia is a hematological malignancy with a five-year survival rate of just 25%. Although hematopoietic stem cell transplantation (HSCT) is offered as salvage therapy for patients with intermediate / high-risk acute myeloid leukemia who have relapsed or drug-resistant disease after chemotherapy, it offers the greatest potential for long-term survival. However, up to 30% of patients will eventually relapse with intermediate-risk leukemia, and high-risk or refractory relapsed leukemia has a higher risk of relapse, so relapse remains the main reason for HSCT treatment failure. Previous studies have observed that T cell depletion is associated with the recurrence rate of AML after HSCT. Howev...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57426G01N33/57488
Inventor 黄晓军赵翔宇刘雪飞
Owner PEOPLES HOSPITAL PEKING UNIV
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