Preparation and application of targeted hypersensitive broad-spectrum oncolytic virus
A recombinant virus, Newcastle disease virus technology, applied in the field of genetic engineering, can solve the problems of drugs with poor efficacy, unsatisfactory tissue-specific expression of gene drugs, poor targeting, etc., to solve the problems of poor efficacy and enhance anti-tumor immune response. Effect
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[0123] Reagents required for the experiment:
[0124] 1. The large plasmid extraction kit is a product of Vazyme, the product number is DC202.
[0125] 2. DMEM is a product of Solarbio Company, the product number is 12100-500ml.
[0126] 3. G-418 is a product of Solarbio, the product number is G8161.
[0127] 4. Opti-MEM is a product of Gibco, the product number is 31985088.
[0128] 5. The transfection reagent is the product of Vazyme Company, the product number is T202-01.
[0129] 6. Cytarabine is a product of McLean, the product number is C805253-5g.
[0130] 7. TPCK is a product of Meilun Biological Company, the article number is MB3477.
[0131] 8. Day-old chicken embryos were purchased from Jinan Saisi Poultry Technology Co., Ltd.
[0132] 9. The RNA extraction kit is a product of Beijing Quanshijin Company, the article number is AT411-02.
[0133] 10. The RT-PCR kit is a product of Beijing Quanshijin Company, the article number is AT411-02.
[0134] experimental...
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