Lagerstroemia terpene synthase gene and application thereof

A technology of terpene synthase and crape myrtle, which is applied in the field of plant genetic engineering and can solve problems such as no research reports on terpene synthase genes.

Pending Publication Date: 2021-11-26
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no research reports on terpene synthase genes in Lagerstroemia genus

Method used

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  • Lagerstroemia terpene synthase gene and application thereof
  • Lagerstroemia terpene synthase gene and application thereof
  • Lagerstroemia terpene synthase gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Cloning and Biological Information Analysis of LCTPS14, LTPS14 of Ziwei Terpene

[0043] Lagerstroemia Caudata is white flowers in Lagerstroemia Caudata and fragrant purple 'Bai Yun Yixia' (L.indica'baiyunyingXIA ') is white flowers, and single material is the main difference volatile ingredients of the flowers of the two. However, in Ziwei, the biological synthesis related genes and functions in Ziwei are still unclear, to analyze the molecular mechanism of the biosynthesis of the Ziwei belly, the present invention performs full length transcription group sequencing, screening to obtain differentially expressed The terpene enzyme gene is based on the tail leafworm and the Ziwei 'Baiyun Yingxia' is a material, the terpene gene, analyzes the expression of the gene, and performs preliminary verification.

[0044] 1, material and method

[0045] 1.1 Plant Material: Refer to Xu Wei (2019) to the study of different parts of the tail leaf Villa and the flowers of the fl...

Embodiment 3

[0134] Example 3 Prokaryotic Expression and Introduction Function of Terpene Olyse Gene

[0135] The present embodiment constructs the prokaryotic expression vector of the target gene LCTPS 14 and LTPS14, which is expressed in E. coli to obtain a recombinant protein, performing in vitro enzymatic reaction with FPP / GPP, GC-MS technology analysis to generate substances, and obtain recombination The function of the protein.

[0136] 1, material and method

[0137] 1.1 vector and strain

[0138] Arctic-Express BL21 (DE3) and BL21 (DE3) PLYSS expression vectors PCZN1, PET30A, PGEX-4T-1 Buy in Nanjing Zhongding Biotechnology Co., Ltd. Escherichia coli feel TOP10 is purchased in Shanghai Online Biotechnology Co., Ltd.

[0139] 1.2 reagent and consumables

[0140] Protein Marker (Thermo), GPP ammonium (Sigma, Cas: 763-10-0, Item No. G6772), FPP ammonium salt (Sigma, Cas: 13058-04-3, IPTG (Sigma, CAS: 367- 93-1), ARC (Sigma, Cas: 79-06-1), BI (Sigma, Cas: 110-26), Tris (Sigma, Cas: 77-86...

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Abstract

The invention discloses a lagerstroemia terpene synthase gene and application thereof. According to the invention, the terpene synthase gene is cloned and separated from lagerstroemia plants for the first time, the catalytic function of the terpene synthase gene is explored through in-vitro expression, the molecular mechanism of biosynthesis of floral substances of the lagerstroemia plants is preliminarily understood, and a basis is provided for further research on floral biosynthesis and regulation of the lagerstroemia plants.

Description

Technical field [0001] The present invention relates to the field of plant gene engineering, and in particular, to the genus gene and its application thereof. Background technique [0002] Lagerstroemia Caudata and the flavoring violet mustache 'Bai Yun Yixia' (L.indica'baiyunyingXIA ') is white flowers, and the monotenic substance is the main difference volatile ingredient of the flowers of the expansion period. The tail leafway is a single aroma characteristic component and the content is higher. The purple 'Baiyun Yingxia' is small and the monotenoids are small and low concentration, and terpene is the synthetic pathway of phytosomal terpene. Key enzyme. There is currently no research report on terpenonase genes in Ziwen plants. Inventive content [0003] It is an object of the present invention to provide a hazelic terpene synthase gene and its application thereof. [0004] For the purposes of the present invention, the present invention provides a gene LCTPS 14 (from the ta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/88C12N15/60C12N15/84C12N15/70C12N1/21A01H5/02A01H6/82C12P7/04
CPCC12N9/88C12N15/8205C12N15/8243C12N15/70C12P7/04C12Y402/03C12N2800/22
Inventor 蔡明章寒张启翔潘会堂朱梓宁续言吴宜静张一鸣王佳程堂仁
Owner BEIJING FORESTRY UNIVERSITY
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