New application of nobiletin and nobiletin composition

A technique for a new use of Chuan Chen Pei, applied in the field of a new use of Chuan Chen Pei and its composition, and can solve the problems of poor curative effect, reduced drug sensitivity, drug resistance and the like

Pending Publication Date: 2021-11-30
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after taking MTX for a long time, it leads to decreased drug sensitivity and drug resistance, resulting in poor curative effect

Method used

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  • New application of nobiletin and nobiletin composition
  • New application of nobiletin and nobiletin composition
  • New application of nobiletin and nobiletin composition

Examples

Experimental program
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Embodiment 1

[0012] 1. Experimental materials

[0013] Bovine type II collagen (Chondrex, Inc), incomplete Freund's adjuvant (Chondrex, Inc), methotrexate was purchased from Toronto Research Chemical Company, nobiletin was purchased from Shanghai Yuanye Co., Ltd. (purity > 99% ). Primary antibody: Anti-P-gp(ab170904), AKT(4691T), p-AKT(4060T), PI3K(4257T), p-PI3K(17366S), PKM2(4053T), HK2(2867T), LDHA(3582T) , HIF-1α (36169T), β-actin (3077T) were purchased from Abcam Company and Cell Signaling Technology Company of the United States. Secondary antibodies were purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. ATP detection kit (H245), GLUCOSE detection kit (A012-1), Lacate detection kit (H093) were purchased from Nanjing Jiancheng Bioengineering Institute. Annexin V-FITC / PI Cell Apoptosis Test Kit (559763) was purchased from BD Company, USA. 10×SDS-PAGE electrophoresis buffer (B1005-500ML), 10×protein electrotransfer buffer (B1006-500ml), and ultra-sensitive luminescence...

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Abstract

The invention belongs to the technical field of medicine preparation, and particularly relates to a new application of nobiletin and a nobiletin composition. The nobiletin is applied to preparation of drugs for reducing P-gp expression in synovial tissues of arthritis. Combined use of nobiletin and methotrexate can reduce cartilage and bone injuries and improve swelling and arthritis injury. On the other hand, nobiletin can reverse P-gp expression in patients with arthritis injury, thereby reducing outflow of the methotrexate and maintaining effect of the methotrexate. The nobiletin and the methotrexate have an obvious synergistic effect. The growth inhibition and apoptosis of RA-FLS/methotrexate cells can be recovered through the joint treatment of the nobiletin and the methotrexate at noncytotoxic concentration of nobiletin. The nobiletin can be used as a potential chemical sensitizer to overcome drug resistance in RA.

Description

technical field [0001] The invention belongs to the technical field of new uses of medicines, and in particular relates to a new use of nobiletin and its composition. Background technique [0002] Rheumatoid arthritis (Rheumatoid arthritis, RA) is a chronic autoimmune disease with multiple joint inflammation as the main clinical manifestation. High (60%-70%), and the prognosis is poor, seriously endangering human life and health. The etiology of RA is not yet clear, but it is generally believed to be related to various factors such as abnormal immune system, heredity, and infection. The basic pathological features of RA patients include synovitis and angiogenesis. On the one hand, synovial blood vessels proliferate and secrete a large number of cytokines, which cause synovial tissue thickening, destroy cartilage and bone structure integrity, and invade soft tissues around bone joints, resulting in joint damage. Malformation, on the other hand, increases angiogenesis and in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P19/02A61P29/00A61P19/08A61K31/519
CPCA61K31/352A61P19/02A61P29/00A61P19/08A61K31/519A61K2300/00
Inventor 刘元艳刘蕊宋钰蓉李晨曦华正来罗欣怡李阳
Owner BEIJING UNIV OF CHINESE MEDICINE
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