Product for inducing antler growth of female deer, preparation method of product, method for inducing antler growth of female deer and application
A product and antler growing area technology, applied to medical preparations containing active ingredients, pharmaceutical formulas, peptide/protein components, etc., can solve problems that cannot be applied to actual production, affect reproductive performance of doe, etc., and increase economic added value , the effect of high velvet growth rate
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[0023] The present invention also provides a preparation method of the above product, which includes the following steps: taking the cytokine and preparing it so that the mass concentration in the product is 1 ng / ml-1 mg / ml. In the present invention, when the ingredients of the product include an emulsifier, the preparation method further includes: emulsifying after mixing the emulsifier and the cytokine. In the present invention, the emulsification method preferably includes two-way syringe emulsification or oscillator vibration emulsification. The two-way syringe emulsification is preferably emulsified more than 200 times, more preferably 300 times; the oscillator emulsification is preferably shaken at 10000 rpm for more than 30 minutes, more preferably 30 minutes.
[0024] The method for quality inspection of the emulsion is: centrifuge the emulsion at 5000g / min for 5 minutes and pass the inspection if there is no emulsion stratification; or drop a drop of the emulsified pr...
Embodiment 1
[0029] Product composition: The cytokines are CCL2 and CCL3, both at a concentration of 100ng / ml.
[0030] Preparation:
[0031] It can be prepared according to the concentration of the above-mentioned cytokine solution, and the diluent is physiological saline.
Embodiment 2
[0033] Product composition: cytokines are CCL2 and CCL3, the concentration is 100ng / ml; emulsifier is liquid paraffin and lanolin, the mass ratio of liquid paraffin and lanolin is 2:1; the volume ratio of cytokines and emulsifier is 1: 1.
[0034] Preparation:
[0035] Mix the emulsifier and cytokine solution according to the above ratio, and emulsify 200 times with a two-way syringe. The emulsion is centrifuged at 5000g / min for 5min, and it is qualified if no emulsion stratification occurs.
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