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Vaccine composition for preventing and controlling porcine reproductive and respiratory syndrome and having broad-spectrum mucosal immunity function and application of vaccine composition

A vaccine composition and a technology for respiratory syndrome, applied in the field of medicine and biology, can solve the problems of aggravation, affect the recovery of the disease, unclear mechanism, etc., achieve the improvement of the immune effect, reduce the risk of allergy and disease transmission, and prolong the immune period. Effect

Inactive Publication Date: 2015-04-29
吕宏亮 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Many studies have reported that the combined use of α-interferon and porcine reproductive and respiratory syndrome virus cannot stimulate strong antiviral immunity in pigs. The virus itself inhibits interferon signal transduction in pigs, and the detailed mechanism is still unclear.
In general, interferon has a broad-spectrum antiviral effect, inhibits virus replication, and may have a negative effect on the level of interferon induced by live virus, but many experiments have shown the effect of interferon on the disease state and process of infected or exposed animals, in other words It is said that it affects the recovery or aggravation of the disease

Method used

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  • Vaccine composition for preventing and controlling porcine reproductive and respiratory syndrome and having broad-spectrum mucosal immunity function and application of vaccine composition
  • Vaccine composition for preventing and controlling porcine reproductive and respiratory syndrome and having broad-spectrum mucosal immunity function and application of vaccine composition
  • Vaccine composition for preventing and controlling porcine reproductive and respiratory syndrome and having broad-spectrum mucosal immunity function and application of vaccine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Inactivation and Purification of Complete Porcine Reproductive and Respiratory Syndrome Virus Antigen and Preparation of Vaccine Composition

[0034] 1. Preparation of porcine reproductive and respiratory syndrome virus antigen

[0035] 1.1 Virus culture

[0036] MARC-145 cells were adapted to serum-free medium, transferred to a bioreactor filled with VP SFM medium (Invitrogen) and microcarrier cytodex 1, and cultured at 37°C for 3-4 days, the pH of the culture medium was 7.2±0.2, and oxygen saturation The temperature is 25±0.1%, and the stirring speed is medium speed. The porcine reproductive and respiratory syndrome virus was infected at 0.01 MOI, and the virus culture fluid was harvested at 7, 11, and 15 days, and the virus fluid was added to infect each harvest.

[0037] 1.2 Clarification

[0038] The harvested liquid was filtered with 8 μm polypropylene pre-filter, and then filtered and clarified through 0.8 μm and 0.2 μm filters, and the virus titer (...

Embodiment 2

[0058] The preparation of embodiment 2 lyophilized purified inactivated vaccines

[0059] 1. Preparation of porcine reproductive and respiratory syndrome virus antigen

[0060] Method is with embodiment 1.

[0061] 2. Preparation of stable synergist (100ml)

[0062] Method is with embodiment 1.

[0063] 3. Vaccine composition preparation

[0064] The obtained porcine reproductive and respiratory syndrome virus whole virus particle antigen is added to the prepared stable synergist, so that the amount of virus protein reaches 25 μg / mL.

[0065] Follow the steps below to lyophilize, freeze at -40°C, and sublimate twice, the first sublimation conditions: temperature -15°C, pressure 80μPa, until the ice is completely sublimated; the second sublimation conditions: temperature 40°C, pressure 80μPa, keep until residual The water content is 2-3%, and the virus loss is not more than 5% during the freeze-drying process. Ready to serve.

Embodiment 3

[0066] The preparation of embodiment 3 lyophilized purified inactivated vaccines

[0067] 1. Preparation of porcine reproductive and respiratory syndrome virus antigen

[0068] Method is with embodiment 1.

[0069] 2. Preparation of stable synergist (100ml)

[0070] To 100ml of 50mM phosphate buffer, add 1g of arginine and glutamic acid mixture in equal weight ratio, 2.0g of Poly ICLC, 8g of fucose, 5g of mannitol, 0.4g of urea, 0.04 gEDTA, 0.01g Tween-20, and adjust the pH value to 8.0.

[0071] 3. Vaccine composition preparation

[0072] The obtained porcine reproductive and respiratory syndrome virus whole virus particle antigen is added to the prepared stable synergist, so that the amount of virus protein reaches 25 μg / mL.

[0073] Follow the steps below to lyophilize, freeze at -40°C, and sublimate twice, the first sublimation conditions: temperature -15°C, pressure 80μPa, until the ice is completely sublimated; the second sublimation conditions: temperature 40°C, press...

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Abstract

The invention discloses vaccine composition for preventing and controlling porcine reproductive and respiratory syndromes and having a broad-spectrum mucosal immunity function and an application of the vaccine composition. The vaccine composition comprises inactivated and purified complete porcine reproductive and respiratory syndrome viral antigen and a stable synergist, wherein the antigen is from HP-PRRSV (high pathetic porcine reproductive and respiratory syndrome virus)-JXM-F5 strain, and the microbial preservation number is CGMCC NO.9453; the stable synergist comprises essential and non-essential amino acid mixtures, a class I interferon inducer, disaccharide, polyhydric alcohol, urea or urea derivatives, EDTA (ethylene diamine tetraacetic acid) or EDTA salt, a surface active agent and a buffer solution. The vaccine composition is highly secure and has a broad-spectrum mucosal immunity protection function; the storage stability of a PRRSV stock solution, a semi-finished product and a finished product in the scale production process is improved, particularly, the antigenic structure integrity can be kept in the extreme temperature environment, titer is prevented from decreasing, and storage and use of PRRSV vaccines in the high temperature areas are facilitated.

Description

technical field [0001] The invention relates to a vaccine composition and its application, in particular to a broad-spectrum mucosal immunity composition for preventing and treating porcine reproductive and respiratory syndrome and its application, belonging to the field of medical biotechnology. Background technique [0002] There are 3 types of cell-cultured virus vaccines: live attenuated vaccines, inactivated whole virus vaccines, and virus subunit vaccines. Depending on the virus, its physical and chemical properties and biological characteristics are different, and the excipients and conditions for preserving its antigens are also different. . To preserve the integrity of inactivated virus or the infectivity of live attenuated virus, it is necessary to develop suitable excipients. Lyoprotectants for live vaccines of varicella zoster, measles, chickenpox and porcine Japanese encephalitis have been developed, and lyoprotectants for inactivated vaccines have been success...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61K39/39A61P31/14C12N7/00C12R1/93
Inventor 张澍吕宏亮
Owner 吕宏亮
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