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Detection method with high detection rate of carbapenem-resistant klebsiella pneumoniae in hospital environment

A kind of technology of Klebsiella pneumoniae and carbapenems

Pending Publication Date: 2022-03-22
昆明医科大学第二附属医院
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Problems solved by technology

[0005] In order to make up for the deficiencies of the existing technology and solve the existing errors in the existing detection methods and the low bacterial content of the target colonies, which are easily affected by other miscellaneous bacteria, resulting in the detection rate of carbapenem-resistant Klebsiella pneumoniae Low problem, the detection method that the detection rate of carbapenem-resistant Klebsiella pneumoniae in the hospital environment proposed by the present invention is high

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  • Detection method with high detection rate of carbapenem-resistant klebsiella pneumoniae in hospital environment
  • Detection method with high detection rate of carbapenem-resistant klebsiella pneumoniae in hospital environment
  • Detection method with high detection rate of carbapenem-resistant klebsiella pneumoniae in hospital environment

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Embodiment approach

[0042] As an embodiment of the present invention, in the S1, the specific steps of sampling are:

[0043]A1: Take a 5cm×5cm sterilization specification plate and place it on the surface of the object to be inspected;

[0044] A2: Take the cotton swab and soak it in the nutrient broth containing neutralizing agent;

[0045] A3: Apply the nutrient broth containing the neutralizing agent back and forth 5 times horizontally and vertically in the specification plate;

[0046] A4: Rotate the cotton test piece, and continuously sample the area of ​​4 standard boards to complete the sampling step.

[0047] As an embodiment of the present invention, in the above-mentioned A4, the sampling area of ​​the cotton test piece is judged according to the collected area, when the collected area2 , take the entire surface; the area to be mined is ≥100cm 2 , take 100cm 2 .

[0048] As an embodiment of the present invention, in said S2, the cotton test sample after sampling needs to be cut off...

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Abstract

The invention belongs to the technical field of clinical microbiological detection, and particularly relates to a hospital environment carbapenem-resistant klebsiella pneumoniae detection rate high detection method, which comprises the following steps: S1, sampling: sampling the surface of a detected object by using a sterilization specification board and a cotton test piece; the carbapenem-resistant klebsiella pneumoniae on the surface of a to-be-detected object is adsorbed by a cotton test piece soaked with nutrient broth, and is cultured in a constant-temperature box at 35 + / -1 DEG C for 24 hours, so that the growth quantity of the carbapenem-resistant klebsiella pneumoniae is greatly increased, and meanwhile, bacteriostats are generated to inhibit the growth of other gram-positive bacteria, so that the detection sensitivity is greatly improved. The carbapenem-resistant klebsiella pneumoniae can be detected through special detection methods in the steps S1 to S5, and different from a traditional detection mode, the detection rate of the carbapenem-resistant klebsiella pneumoniae is increased, a basis is provided for prevention and control work of hospitals, the occurrence rate of the carbapenem-resistant klebsiella pneumoniae in the hospitals is reduced, and the clinical application prospect is broad. And the workload is reduced to a certain extent.

Description

technical field [0001] The invention belongs to the technical field of clinical microorganism detection, in particular to a detection method with high detection rate of carbapenem-resistant Klebsiella pneumoniae in hospital environment. Background technique [0002] The cleaning and disinfection of hospital environment plays a key role in reducing the number of bacteria on the surface of environmental objects and reducing the rate of nosocomial infection. The quality of cleaning and disinfection of the surface of environmental objects is closely related to the occurrence of nosocomial infection. [0003] In recent years, with the increase of Klebsiella pneumoniae's resistance to carbapenems, it has brought great challenges to clinical treatment. An important source of infection and transmission medium for transmission and outbreaks. Environmental cleaning and disinfection play an important role in reducing the risk of transmission of nosocomial infection by carbapenem-resis...

Claims

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Application Information

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IPC IPC(8): C12Q1/10C12Q1/24C12M1/00C12R1/22
CPCC12Q1/10C12Q1/24C12M23/22C12M23/46C12M23/48C12M23/52C12M37/04G01N2333/26
Inventor 杨旭单斌李庆蓉
Owner 昆明医科大学第二附属医院
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