Phytophthora nicotianae inoculation method applicable to simple and rapid biocontrol effect verification

A technology of Phytophthora tabacum and Phytophthora tabacum, applied in the direction of microorganism-based methods, biochemical equipment and methods, tobacco cultivation, etc., can solve the problems of less procedure steps, non-uniform inoculation amount, and large incidence of inoculation amount, and achieve program The effect of less steps and prolonging the experimental cycle

Pending Publication Date: 2022-03-25
TOBACCO RES INST HENAN ACADEMY OF AGRI SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a procedure with few steps, which is relatively simple and time-saving, and solves the problem of inconvenient observation of the onset of Phytophthora nicotiana in the prevention effect verification experiment. The big onset is too fast, which is not conducive to observation and recording

Method used

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  • Phytophthora nicotianae inoculation method applicable to simple and rapid biocontrol effect verification
  • Phytophthora nicotianae inoculation method applicable to simple and rapid biocontrol effect verification

Examples

Experimental program
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Effect test

Embodiment 1

[0023] 1. Preparation of potted tobacco seedlings: select tobacco seedlings (4-6 leaves, variety: China Tobacco 100) that grow uniformly after 30 days of conventional seeding and transplant them into small flower pots filled with pure substrate with a diameter of 8.5 cm and a height of 7 cm. 5 biocontrol treatments, 1 control treatment, 10 pots of tobacco seedlings for each treatment.

[0024] 2. Phytophthora nicotiana solution preparation: Phytophthora nicotiana was inoculated on OA solid medium plate and cultured at 25° C. for 5 days. Punch holes with a 5mm hole puncher, inoculate 5 pieces of punched mycelium into OA medium per 300mL liquid (500mL Erlenmeyer flask), 28°C, 180r﹒ Shake in a min-1 shaker for 7 days. Ordinary soybean milk machine homogenizes for 10 seconds.

[0025] 3. Inoculation: 5mL of Phytophthora nicotiana solution is applied to the root of each tobacco seedling, and the biocontrol agent is used to treat the corresponding required bacterial agent for root...

Embodiment 2

[0032] 1. Preparation of potted tobacco seedlings: select tobacco seedlings (4-6 leaves, variety: China Tobacco 100) that grow uniformly after 35 days of conventional seeding and transplant them into small flower pots filled with pure substrate, 10 cm in diameter and 8.5 cm in height. 4 chemical treatments, 1 control treatment, 10 pots of tobacco seedlings for each treatment.

[0033] 2. Phytophthora nicotiana solution preparation: Phytophthora nicotiana was inoculated on OA solid medium plate and cultured at 25° C. for 5 days. Punch holes with a 5mm hole puncher, inoculate 5 pieces of punched mycelium into OA medium per 300mL liquid (500mL Erlenmeyer flask), 28°C, 180r﹒ Shake in a min-1 shaker for 7 days. Ordinary soybean milk machine homogenizes for 10 seconds.

[0034] 3. Inoculation: 1mL of Phytophthora nicotiana liquid is applied to the roots of each tobacco seedling, and the biocontrol agent is used to treat the corresponding requirements for root irrigation. Then cul...

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Abstract

The invention relates to the field of phytopathogen inoculation, in particular to a phytophthora nicotianae inoculation method which is suitable for simple and rapid biocontrol effect verification, and aims to solve the problem that the disease condition is inconvenient to observe after phytophthora nicotianae is inoculated back in a control effect verification experiment, and the existing method is not uniform in inoculum size, high in inoculum size, too fast in disease and not beneficial to observation and recording. According to the method, through an inoculum size gradient experiment, the inoculum size with the moderate incidence speed of the inoculated tobacco seedlings is screened out, and observation and recording of the incidence condition of the tobacco plants are facilitated; in addition, the method is few in procedure steps, simple, convenient and time-saving, and the steps in the prior art are relatively numerous, labor-consuming and time-consuming.

Description

technical field [0001] The invention relates to the field of plant pathogenic bacteria inoculation, in particular to a simple and quick Phytophthora nicotiana inoculation method suitable for biocontrol effect verification. Background technique [0002] Phytophthora nicotianae is the pathogen of tobacco black shank. Tobacco black shank is a major rhizome disease in tobacco production (Wang Fenglong, Zhou Yihe, Ren Guangwei. Illustrated Handbook of Tobacco Diseases in China [M]. Beijing: China Agricultural Press, 2019.). Therefore, it is very important to prevent and control tobacco black shank in tobacco fields. In addition to conventional chemical control, biological control is currently a research hotspot in tobacco disease control because of its high safety. Biological control is inseparable from the screening of bio-control bacteria, and the screened bio-control bacteria need to verify the bio-control effect of indoor potted plants. During the verification experiment, ...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01G22/45C12N1/14C12R1/645
CPCA01G7/06A01G22/45C12N1/14
Inventor刘畅李小杰李成军姚晨虓苗淑月王雪芬李洪亮白静科邱睿陈玉国赵钧李淑君
OwnerTOBACCO RES INST HENAN ACADEMY OF AGRI SCI