Liver cancer model cell, in-vitro culture method, application and liver cancer animal model establishment method

Abstract

The invention discloses a liver cancer model cell, an in-vitro culture method, application and a liver cancer animal model establishment method. According to WB expression detection, by taking beta-actin as a reference, the relative expression quantity of HAb18G / CD147 is 3.6 to 6.07, the relative expression quantity of paxillin is 4.39 to 6.97, and the relative expression quantity of FAK is 1.85 to 3.17. The invention further discloses a preparation method of the liver cancer model cell. The liver cancer model cell is transplanted into a mouse body, an established liver tumor model mouse is high in tumor proliferation, adhesion and invasion ability, the anti-apoptosis ability is enhanced, and the liver cancer model cell is very suitable for establishing a typical animal model for tumor drug screening and has very important practical significance in targeted tumor drug development.

Description

technical field [0001] This application relates to the technical field of liver cancer models, in particular to liver cancer model cells, in vitro culture methods, applications, and liver cancer animal model establishment methods Background technique [0002] In a large number of liver cancer drug research, it is particularly important to establish a rat model of liver cancer. Commonly used rat liver cancer models are divided into transplantation type and primary type. [0003] The primary liver cancer model uses chemical carcinogens to induce the slow development of liver cancer in rats. Although this model takes a long time, the main stages of liver cancer development can be observed, which is conducive to the detection of early precancerous lesions of liver cancer. At present, there are many models of primary rat liver cancer induced by DEN at home and abroad. The main problem of this model is that the exposure dose is inaccurate and the time is long. In addition, with...

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Problems solved by technology

The main problem of this model is that the dose of poison is not accurate and the time is long

However, the existing transplantation models have a short tumor formation time, but the process of liver cancer cannot be observed, and the tumor enhancement, adhesion and invasion abilities of the model animals are also average, which is not conducive to comprehensive research on tumor cell drug resistance.

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