Serum miRNA combination for thyroid tumor diagnosis and 131 iodine treatment and prognosis

A thyroid cancer and auxiliary diagnosis technology, applied in the field of biomedicine, can solve problems such as death and insensitivity of patients to treatment

Pending Publication Date: 2022-05-06
ACADEMY OF MILITARY MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still a small number of patients with early progressive metastases. 131 I treatment is not sensitive, even death, etc.

Method used

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  • Serum miRNA combination for thyroid tumor diagnosis and 131 iodine treatment and prognosis
  • Serum miRNA combination for thyroid tumor diagnosis and 131 iodine treatment and prognosis
  • Serum miRNA combination for thyroid tumor diagnosis and 131 iodine treatment and prognosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Embodiment 1, serum miRNA is used for diagnosing thyroid cancer

[0078] Collect the blood of normal people (25 cases), benign thyroid disease patients (10 cases) with known clear pathological information, and thyroid cancer patients (25 cases), put the blood into a centrifuge tube, and centrifuge at 1500-2000 rpm for 10 minutes , take the upper serum.

[0079] RNA extraction: Total RNA was extracted from serum according to the instruction of Trizol kit (Trizol kit can be obtained from commercial sources).

[0080] Poly(A) tailing: Carry out tailing reaction according to the instructions of poly(A) polymerase, the system is shown in Table 1 below (tailing reagents can be obtained from commercial sources).

[0081] Table 1, poly(A) tailing reaction system and conditions

[0082]

[0083] Note: The above system can be scaled up.

[0084] Reverse transcription: the above-mentioned tailed product was used as a template for reverse transcription reaction, and the syste...

Embodiment 2

[0112] Example 2. Serum miRNA is used to evaluate the prognosis of patients with thyroid cancer treated with 131 iodine

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Abstract

The invention discloses a serum miRNA combination for thyroid tumor diagnosis and 131 iodine treatment and prognosis. The application of the substance for detecting miRNA in preparation of a product with any one of the following functions: diagnosing thyroid cancer, screening thyroid cancer, distinguishing thyroid cancer from thyroid benign lesion, distinguishing thyroid cancer from healthy people, evaluating prognosis conditions of thyroid cancer patients after 131 iodine treatment, and predicting survival conditions of the thyroid cancer patients after 131 iodine treatment. The miRNA is Has-miR-378d, Has-miR-130b-5p, and / or Has-miR-548j-3p, and the miRNA is characterized in that: Has-miR-378d, Has-miR-130b According to the serum miRNA marker explored by the invention, rapid early diagnosis of thyroid cancer and prejudgment of 131I treatment effect and survival prognosis condition can be realized, so that clinical doctors are guided to provide prevention and treatment schemes for patients.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a group of serum miRNA combinations used for thyroid tumor diagnosis, 131 iodine treatment and prognosis. Background technique [0002] Thyroid cancer is a common malignant tumor of the endocrine system, accounting for 1%-1.5% of all malignant tumors, and it is on the rise worldwide. The clinical diagnosis of thyroid cancer is a hot issue in clinical work at this stage. Papillary thyroid carcinoma and follicular carcinoma are the most common thyroid cancers (accounting for 90%). Radical surgery and TSH suppression therapy are given clinically, and 131 iodine ( 131 1) radiation therapy, the ten-year survival rate reaches more than 90%. However, there are still a small number of patients with early progressive metastases. 131 I treatment insensitivity, and even death. Thyroid cancer does not have any clinical symptoms in the early stage. Most clinically, the nature of thyroid nodules ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11C12Q1/6851G16B30/00G16H50/20
CPCC12Q1/6886C12Q1/6851G16B30/00G16H50/20C12Q2600/178C12Q2600/118C12Q2600/166C12Q2600/158C12Q2563/107C12Q2531/113
Inventor 葛常辉丁勇刘娜郑晓飞陈方旎付汉江朱捷
Owner ACADEMY OF MILITARY MEDICAL SCI
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