Method for cultivating high-quality tussah cordyceps militaris

A cultivation method and technology of tussah silkworm chrysalis, applied in the field of edible fungus cultivation, can solve the problem of high selling price, and achieve the effects of reducing the price of raw materials and improving the quality and economic benefit.

Pending Publication Date: 2022-05-13
LIAONING OCEAN & FISHERIES SCI RES INST
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Problems solved by technology

Since protein is an important component of cells, the traditional concept tends to choose live female tussah silkworm pupae with high protein content as the host to cultivate tussah silkworm Cordyceps militaris. The tussah chrysalis are a mixture of male and female tussah chrysalis, and the quality of the bred tussah chrysalis remains to be further improved

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  • Method for cultivating high-quality tussah cordyceps militaris

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Embodiment Construction

[0008] A high-quality breeding method of tussah silkworm Cordyceps militaris of the present invention is to inoculate the liquid strain of Cordyceps militaris in live tussah silkworm pupae to cultivate fruiting bodies according to the method of the prior art. The live pupae of male autumn tussah silkworm obtained by relieving diapause were used.

[0009] The specific method is as follows:

[0010] 1. Preparation of Cordyceps militaris liquid culture medium

[0011] Medium formula: Potato 200g Glucose 20g Potassium dihydrogen phosphate 3g Magnesium sulfate 1.5g Vitamin B 2 10mg peptone 3g beef extract 5g water 1000ml pH value 7; sterilize by autoclaving at 121°C, keep it for 30 minutes after the pressure reaches 0.1-0.11MPa, and then use it for later use.

[0012] 2. Preparation of Cordyceps militaris liquid strain

[0013] Take 0.3 cm of the "Bingyu No. 1" Cordyceps militaris test-tube mother seed bred and recorded by Dalian Institute of Biotechnology, Liaoning Academy of A...

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Abstract

The invention discloses a method for cultivating high-quality tussah silkworm cordyceps militaris, which comprises the following steps: inoculating cordyceps militaris liquid strains into tussah silkworm live pupae to cultivate sporocarp, and completely adopting male autumn tussah silkworm live pupae obtained by diapause relief. The production rate of the product of the tussah cordyceps militaris reaches 90% or above, and compared with the fact that female autumn tussah live pupae with the same mass are completely adopted, the content of cordycepin in the product is increased by 43.02%, the content of adenosine is increased by 28.12%, the content of cordyceps polysaccharide is increased by 20.76%, meanwhile, the price of raw materials of the product with the same mass is greatly reduced, and the quality and economic benefits of the tussah cordyceps militaris are remarkably improved.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a method for cultivating high-quality tussah silkworm Cordyceps militaris. Background technique [0002] Cordyceps militaris ( cordy Cep Smilitaris (L.) Link ), also known as Cordyceps militaris, is a model species of the genus Cordyceps of the Ascomycotina ergotaceae. It belongs to a different species from Cordyceps sinensis. , anti-tumor and improve myocardial function. [0003] Tussah Cordyceps militaris is the fruiting body grown by inoculating the wild Cordyceps militaris fungus into live tussah silkworm pupae after isolation and domestication. From the analysis of its active ingredients, it not only contains nutrients such as protein and fat, but also contains active ingredients such as cordycepin, adenosine and cordyceps polysaccharide. Cordycepin is a unique nucleoside active substance in Cordyceps sinensis, which has anticancer, antibacte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 都兴范李亚洁温志新李学军陈星合孟楠米锐王林美李冬梅
Owner LIAONING OCEAN & FISHERIES SCI RES INST
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