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Application of Onalspib in preparation of medicine for preventing and/or treating adenovirus infection

An adenovirus and drug technology, applied in the field of medicine, can solve problems such as no adenovirus infection found, and achieve the expected effects of good safety, good safety, and short medication cycle

Pending Publication Date: 2022-07-22
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report on the treatment of adenovirus infection with Onalespib

Method used

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  • Application of Onalspib in preparation of medicine for preventing and/or treating adenovirus infection
  • Application of Onalspib in preparation of medicine for preventing and/or treating adenovirus infection
  • Application of Onalspib in preparation of medicine for preventing and/or treating adenovirus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Evaluation of cytotoxicity of Onalespib in Vero cell line

[0040] 1. Cell culture

[0041] After 2 passages of cryopreserved and resuscitated cells, use DMEM medium containing 10% fetal bovine serum and double antibody (penicillin 100U / mL, streptomycin 100μg / mL) for expansion and culture, and the seeding density is not less than 1 ×10 4 cell / mL, passage density not higher than 5×10 4 cells / mL.

[0042] 2. Drug treatment of cells

[0043] Vero cells by 1 x 10 4 Cells / well (volume 100 μL) were seeded in 96-well cell culture plates, and cultured for 24 hours until the confluence of cell wells reached 80%; 200 μL of culture medium (DMEM medium + 2% serum + double antibody) was used in each well to prepare drugs, and Add to the corresponding cell wells and mix. Seven drug concentration gradients were set in the drug treatment group, and each gradient concentration was set up with 2 duplicate wells, and the final concentrations were 0.007 μM, 0.02 μM, 0.06 μ...

Embodiment 2

[0048] Example 2: Evaluation of anti-AdV3 adenovirus activity of Onalespib in Vero cell line

[0049] 1. Cell culture

[0050] After 2 passages of cryopreserved and resuscitated cells, use DMEM medium containing 10% fetal bovine serum and double antibody (penicillin 100U / mL, streptomycin 100μg / mL) for expansion and culture, and the seeding density is not less than 1 ×10 4 cell / mL, passage density not higher than 5×10 4 cells / mL.

[0051] 2. Drug treatment of cells

[0052] Vero cells by 1 x 10 4 Cells / well (volume 100 μL) were inoculated into 96-well cell culture plates, and cultured for 24 hours until the confluence of cell wells reached 80%; AdV3 virus of 0.55MOI (multiplicity of infection) was added to both the drug-treated group and the virus control group, while the drug-treated group Add each gradient concentration of the drug (with 5 μM as the starting concentration, 7 gradients of serial 3-fold serial dilution, two duplicate wells for each gradient) to a total vol...

Embodiment 3

[0062] Example 3: Evaluation of anti-AdV5 adenovirus activity of Onalespib in Vero cell line

[0063] 1. Cell culture

[0064] After 2 passages of cryopreserved and resuscitated cells, use DMEM medium containing 10% fetal bovine serum and double antibody (penicillin 100U / mL, streptomycin 100μg / mL) for expansion and culture, and the seeding density is not less than 1 ×10 4 cell / mL, passage density not higher than 5×10 4 cells / mL.

[0065] 2. Drug treatment of cells

[0066] Vero cells by 1 x 10 4 Cells / well (volume 100 μL) were inoculated in 96-well cell culture plates, and cultured for 24 hours until the confluence of cell wells reached 80%; 1.1 MOI (multiplicity of infection) AdV5 virus was added to the drug treatment group and the virus control group, while the drug treatment group Add each gradient concentration of the drug (with 5 μM as the starting concentration, 7 gradients of serial 3-fold serial dilution, two duplicate wells for each gradient) to a total volume of...

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to application of Onalspib in preparation of a medicine for preventing and / or treating adenovirus infection. The invention discloses an application of Onalspib in preparation of a medicine for inhibiting adenovirus and / or a medicine for preventing and / or treating adenovirus infection, so that a safe and effective small molecular compound is provided for clinically treating adenovirus. The Onalspib can effectively inhibit the replication of the adenovirus in a non-toxic range, can be further developed into a medicine for treating or preventing diseases caused by adenovirus infection, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more particularly relates to the application of Onalespib in the preparation of a drug for inhibiting adenovirus and / or a drug for preventing and / or treating adenovirus infection. Background technique [0002] Human adenovirus (HAdV) belongs to the mammalian genus Adenovirus of the family Adenoviridae. Adenovirus is a non-enveloped DNA virus with an icosahedral nucleocapsid. The core of the viral genome is a linear double-stranded DNA molecule, about 36kb, and its genome is highly concentrated and packaged, and is packaged by hundreds of similar The histone protein VII and the protamine-like protein Mu (also known as protein X) organize into chromatin. The capsid of adenovirus has 240 hexons, and the 12 tips of the icosahedral capsid are complexes composed of penton and fiber. Twelve fiber proteins protrude from the capsid surface with penton protein as the base, and the top of the fiber f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/496A61P31/20
CPCA61K31/496A61P31/20
Inventor 陈绪林吴建国刘敏丽
Owner JINAN UNIVERSITY
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