63 results about "Adenovirus diseases" patented technology

The invention provides a dual PCR detection probe and kit for duck adenovirus 2 and duck adenovirus A, and belongs to the field of epizootiology. Two pairs of primers which are shown in SEQ ID NO.1-4 are adopted, a dual PCR detection method through which differential diagnosis can be conducted on duck adenovirus 2 and duck adenovirus A in a duck flock is built, a basis is laid for developing an epidemiological survey of adenovirus infection types and scientific prevention and control of relevant diseases in the duck flock, and thus the dual PCR detection probe and kit for duck adenovirus 2 and duck adenovirus A have very important study significance.

The invention relates to preparation and application of a gene chip capable of detecting adenoviruses in a parting mode.A preparation method comprises the steps of preparing specific primers and probes for different types of adenoviruses, preparing oligonucleotides chips, establishing a plurality of PCR systems, and establishing a hybridization system.The gene chip prepared through the method can screen different types of adenoviruses including a 3-type adenovirus, a 7-type adenovirus, a 14-type adenovirus, an 11-type adenovirus and a 55-type adenovirus.The gene chip has the advantages of quickness, accuracy, high throughput and high specificity.A new detection means is provided for clinical diagnoses of different types of adenovirus infections and epidemiological surveys.

The present invention provides methods and compositions for conferring tumor selective cell death on cancer cells expressing specific target precursor messenger RNA molecules (cancer cell selective target pre-mRNAs). The compositions of the invention include conditionally replicative adenoviruses that have been genetically engineered to express one or more pre-trans-splicing molecules (PTMs) designed to interact with one or more cancer cell target pre-mRNA and mediate a trans-splicing reaction resulting in the generation of novel chimeric RNA molecules (chimeric RNA) capable of encoding adenovirus specific protein(s). Adenovirus specific proteins include those proteins complementing an essential activity necessary for replication of a defective adenovirus. The methods and compositions of the invention may be used to target a lytic adenovirus infection to cancer cells thereby providing a method for selective destruction of cancer cells. In addition, the adenoviruses of the invention may be engineered to encode PTMs designed to interact with target pre-mRNAs encoded by infectious agents within a cell, thereby targeting selective destruction of cells infected with such agents.

The invention provides a detection test paper strip of a colloidal gold method for adenovirus IgM and IgG antibodies, a kit and a preparation method thereof. The invention can determine the IgM and IgG antibodies by an immunocapture method principle, and can co-detect specific anti-Adv IgM and IgG antibodies by once operation, thereby simplifying an operation process. The kit is used for simple, rapid and accurate detection, reduces a lot of complex programs, needs neither a special instrument nor special training, is particularly suitable for a primary department, and can make an auxiliary diagnosis effect for early stage or mid-stage adenovirus infection.

The invention relates to a preparation method for obtaining blood used for preparing homologous antiserums and a spleen byproduct used for preparing transfer factors from the bodies of fur bearing animals such as foxes, raccoon dogs and minks; the furs of the foxes, the raccoon dogs and the minks are taken concentratedly in December and confirmed according to the fur-taking dates of different areas; healthy individuals are picked up 45 days before a predicated fur-taking date; the booster immunization of univalent vaccines or polyvalent vaccines of canine distemper, Canine parvovirus enteritis, adenovirus disease, corona virus laxness, parainfluenza, and pasteurellosis is carried out for 3 times by purified and condensed antigens on the foundation of carrying out immunization for once in summer; when the furs are taken, aseptic anticoagulation blood collection is carried out to a heart to improve the blood serum yield; and the spleens of the animals are collected for extracting specific or common transfer factors simultaneously. The blood can be conveniently prepared into the blood serums which resist communicable diseases, and the transfer factors can be extracted the spleen, thus providing guarantee for the healthy development of the breeding in the next year; the invention is used for curing the corresponding communicable diseases of the wild animals of the same species; and when a small amount of communicable disease cases appear in a breeding crowd, the corresponding pathogeny hyper-immune serums and transfer factors are mainly injected to a supposed healthy crowd after the pathogeny is confirmed so as to cure the affected animals in the early period of disease and control the spreading of the epidemic situations.

An assay method for determining the effect of Product R on virus infection of Hela cells. The method comprising the following step: (1) dividing Hela cells into several groups, (2) treating one group with Product R prior to infecting the cells with a virus and treating another group with Product R after the cells being infected with the virus, and (3) determining the effects of Product R on virus infection by comparing the changes in the cell cycle, DNA fragmentation and p53 protein in cells undergone the different treatments in step (2).

The invention relates to a method of inhibiting adenovirus replication. The method comprises the step of contacting Product R with cells that are infected with adenovirus. The invention provides a basis for the treatment of adenovirus infections in human such as pink eye disease.

A method for the therapeutic and prophylactic treatment of adeviruses, More specifically, a method for the therapeutic treatment of adenovirus in a subject by topically administering an antiviral effective amount of CTC-96 to the subject. In addition, a method for the prophylactic treatment against an adenovirus infection in a subject by topically administering a prophylactically anti-adenovirus effective amount of CTC-96 to the subject to minimize the likelihood of the subject veing infected by the adenovirus.

The invention discloses a monoclonal antibody 3-3E for a human adenovirus type 7 and an application of the monoclonal antibody 3-3E. The monoclonal antibody comprises a heavy chain variable region anda light chain variable region, wherein the heavy chain variable region comprises three complementary determining regions HCDR1, HCDR2 and HCDR3; the light chain variable region comprises three complementary determining regions LCDR1, LCDR2 and LCDR3; HCDR1, HCDR2 and HCDR3 are sequentially represented as 26-33-posiition, 51-58-poisiton and 97-110-position from the N end of a sequence 2 in a sequence table; LCDR1, LCDR2 and LCDR3 are sequentially represented as 27-32-posiition, 50-52-poisiton and 89-96-position from the N end of a sequence 4 in a sequence table. On the basis of the clinical demand, the antibody 3-3E resistant to the human adenovirus type 7 is discovered, can be used for preventing and treating adenovirus infection and has great biological and medical significance.

The invention relates to an adenovirus antigen prepared by culturing an adenovirus strain with the preservation number of CGMCC No.9596. The invention also relates to an adenovirus IgM antibody detecting kit. The kit comprises the adenovirus antigen. Compared with the prior art, the diagnostic kit applied to preparation of the adenovirus antigen has the advantages of high specificity, high sensitivity, low cost and the like; the requirements on clinical diagnosis of adenovirus infection are well met.

The present invention provides methods and reagents for detecting antibodies to adenovirus. In a preferred embodiment, the present invention is useful for detecting antibodies to adenovirus serotype 5. In a further preferred embodiment, the present invention can be used in a biosensor-based assay. It is contemplated that the present invention is useful to detect antibodies for ascertaining adenovirus infection, evaluating patient response to gene therapy using adenovirus vectors, developing vaccines to adenovirus infection, developing therapeutics for inducing passive immunity to adenovirus infection, as well as other uses.

The invention relates to a lymphocyte culture method, and in particular discloses a culturing method of adenovirus gene-modifying tumor specificity cytotoxic T lymphocyte. The method comprises: taking 150-200ml of bleeding of the umbilicus or autologous peripheral blood for single karyocyte separation, separately culturing DC cells by adhering to a wall for 2h, and performing T lymphocyte preculture on the non-adhered cells in an IL-2-contained culture medium; collecting mature DC after the DC cells are mature through induction of iAPA factor adenovirus infection, stimulating the autologous primary T lymphocyte to differentiate into cytotoxic T lymphocyte according to an effect target ratio of DC to T being 1 to 10, and observing and recording multiplication capacity of CTL and detecting the cytotoxicity of the CTL. The limitation of a blood component single sampling machine is solved, collected blood volume is less, burden of a patient is reduced, and iAPA-DC can be observed in vitro to stimulate and differentiate the primary T lymphocyte into tumor specificity cytotoxic T lymphocyte.

The invention disclose a novel cairna moschata adenovirus strain, a novel cairna moschata adenovirus inactivated vaccine, and a preparation method for the inactivated vaccine. The adenovirus strain iscollected in the China Center for Type Culture Collection in the Wuhan University on the July 30th, 2019 with a collection number of CCTCC No:V201952. The invention establishes a preparation method for a corresponding inactivated vaccine on the basis of the novel cairna moschata adenovirus. The inactivated vaccine prepared with the method can effectively prevent and treat a novel cairna moschataadenovirus disease, has a good commercial development prospect, solves the problem that no effective solutions can be provided when the novel cairna moschata adenovirus begins to spread on a large scale at present, and greatly reduces the economic loss of cairna moschata breeding industry due to the novel cairna moschata adenovirus disease.

The invention discloses a preparation for restraining adenovirus infection. The preparation is applied to 1 preparing a product for restraining adenovirus infection and 2 restraining adenovirus infection. The preparation is mainly formed by mixing epigallocatechin gallate, tannin and astragalus polysaccharide. The mass ratio of epigallocatechin gallate to tannin to astragalus polysaccharide is (0.5-1.0):(0.5-1.0):(0.5-1.5). The cytotoxicity of the complex formula preparation is not higher than that of a control sample ribavirin obtaining security permission, and the preparation is safe. Under safe use concentration, type 5 adenovirus infection can be effectively restrained through preventive use of the preparation, and the preparation has commercial value for being further developed into an adenovirus infection inhibitor.

The invention discloses a recombinant adenovirus for expressing African swine fever virus B602L-B646L protein and a construction method thereof, and belongs to the technical field of genetic engineering. A recombinant adenovirus shuttle vector pENTRE-EGFP-TOPO is utilized, and a recombinant adenovirus vector pAD-CMV-EGFP-B602L-B646L is obtained through a series of intermediate processes; according to the present invention, the African swine fever virus B602L-B646L protein expression recombinant adenovirus is constructed, the African swine fever virus B602L-B646L protein expression recombinant adenovirus is linearized and transfected with AD293 cells, the recombinant virus is screened according to the cytopathic effect formed by adenovirus infection, the adenovirus packaging process is achieved, the recombinant adenovirus expressing the African swine fever virus B602L-B646L protein is obtained, and the foundation is laid for the construction of the recombinant adenovirus vaccine expressing the African swine fever virus

The invention relates to the technical field of genetic engineering vaccines, and particularly discloses an I-group 4-type fowl adenovirus fiber-2 protein antigen as well as a method for preparing a genetic engineering subunit vaccine and application of the I-group 4-type fowl adenovirus fiber-2 protein antigen. The method comprises the following steps: adding a non-coding gene in front of a fiber-2 gene of the fowl adenovirus FAdV-4, cloning the non-coding gene to a prokaryotic expression vector pET-28a, transforming the prokaryotic expression vector pET-28a to escherichia coli to construct an engineering bacterium, and inducing the engineering bacterium to express to obtain the soluble fiber-2 protein. Wherein the amino acid sequence of the aviadenovirus fiber-2 antigen protein is SEQ ID NO.2, the nucleotide sequence of a gene coded by the aviadenovirus fiber-2 antigen protein is SEQ ID NO.1, and soluble protein with high expression quantity is obtained through non-coding gene regulation and control. The fiber-2 protein of the fowl adenovirus is expressed through escherichia coli, a soluble expression product is obtained, the subunit vaccine is prepared, safety and effectiveness are achieved, and fowl I-group 4-type adenovirus infection can be prevented.

The invention relates to a method for detecting type-5, type-7 and type-55 adenoviruses by using a mass spectrum multiple reaction monitoring technology. The method comprises the following specific steps: synthesizing specific peptide fragment genes of hexon proteins of type-5, type-7 and type-55 adenoviruses; preparing a labeled peptide fragment mixed reagent; determining an ion peak value of the diagnostic marker; preparing the kit; and detecting the sample. The invention provides a new marker for early warning and diagnosis of adenovirus type, type 7 and type 55 infection, and has important clinical value. A mass spectrum result is utilized to confirm that mass spectrum ion peaks consistent with the standard and the heavy standard of preset adenovirus type 5, type 7 and type 55 hexon protein symbolic peptide fragments appear in a serum sample of a patient in the early stage of adenovirus infection. The SILAC-labeled winning and heavy-labeled hexon protein standard mixed reagent of adenovirus type 5, type 7 and type 55 can be used as an early warning and diagnosis marker for adenovirus type, type 7 and type 55 infection, and the result is accurate.