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Egg yolk antibody of type-2 duck adenovirus infection and preparation method

A yolk antibody and adenovirus technology, applied in the direction of antibodies, antiviral agents, chemical instruments and methods, etc., can solve the problems of economic loss, inability to timely and rapid treatment, and difficulty in virus removal in the muscovy duck breeding industry, and achieve prevention and treatment. Infection, good commercial development prospects, good safety effect

Active Publication Date: 2017-11-21
SHANDONG SINDER TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once the virus is brought into the duck flock, it is difficult to remove it, causing serious economic losses to the Muscovy duck breeding industry
[0003] At present, there are only commercial inactivated vaccines to prevent this disease at home and abroad. On the one hand, it takes a long time to produce antibodies after immunization, the protection rate is low, and the economic value is high; on the other hand, when ducks get sick, they cannot be treated promptly and quickly.

Method used

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  • Egg yolk antibody of type-2 duck adenovirus infection and preparation method
  • Egg yolk antibody of type-2 duck adenovirus infection and preparation method
  • Egg yolk antibody of type-2 duck adenovirus infection and preparation method

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Experimental program
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Effect test

Embodiment 1

[0023] 1. Screening of GD strain In 2015, the inventors successfully isolated a duck type 2 adenovirus from a duck group in a duck farm in Guangdong. After PCR detection and Hexon sequencing, the nucleotide sequence is SEQ ID NO: 1, and the encoded amino acid sequence is SEQ ID NO: 2. Through NCBI BLAST comparison analysis, the isolated Hexon gene belongs to the same branch as group I avian adenovirus, and the nucleotide and amino acid homology with the GR strain (accession number: NC_024486.1) in the GenBank database is 99.02% and 99.70, respectively. %. The amino acids at positions 71, 160, 177, 213, 598, and 634 encoded by Hexon of the GD strain and the GR strain are different. The amino acids encoded by the above six positions of the GD strain are valine (V), Alanine (A), alanine (A), glutamic acid (E), threonine (T), isoleucine (I), the amino acids encoded by the above six positions of the GR strain are different Leucine (I), Threonine (T), Valine (V), Arginine (R), Aspa...

Embodiment 2

[0038] Production of refined egg yolk antibody

[0039] 1 The sampling inspection of duck farms for the production of high immunity duck eggs should be free of duck plague, duck viral hepatitis, gosling plague, muscovy parvovirus, avian paramyxovirus and avian influenza, and timely inoculation of viruses and bacteria according to scientific immunization procedures vaccine. Add antibacterial drugs to the feed to prevent bacterial infections and add anticoccidial drugs to prevent coccidia infections.

[0040] 2 Immunization program

[0041] The first immunization: 2 to 3 weeks before delivery, 1.0ml / head;

[0042] Second immunization: 2 weeks after the first immunization, booster immunization is 1.0ml / head;

[0043] The third immunization: 2 weeks after the second immunization, booster immunization is 1.0ml / mouse;

[0044] Fourth immunization: 2 weeks after the third immunization, booster immunization is 1.0ml / head;

[0045] Nth immunization: After the N-1th immunization, every 6-8 weeks,...

Embodiment 3

[0061] Yolk antibody safety test

[0062] 1 Take 5 healthy and susceptible young Muscovy ducklings at the age of 1 day, each subcutaneously inject 2ml of this product, and observe daily for 14 days. There is no death or obvious local reaction or systemic reaction caused by injection of this product.

[0063] 2 Use 5 mice weighing 18-22g, each subcutaneously inject 0.5ml of this product, observe for 7 days, all mice should be healthy.

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Abstract

The invention provides an egg yolk antibody of type-2 duck adenovirus infection. An antigen used in a preparation process of the egg yolk antibody is a vaccine prepared by inactivated GD strain viruses. The type-2 duck adenovirus GD strain has been deposited in China Center for Type Culture Collection in Wuhan University on June 5, 2016 with the accession number of CCTCC No: V201633. The egg yolk antibody of type-2 duck adenovirus infection is good in safety, and any local or systemic adverse reactions caused by the egg yolk antibody do not exist. By identification, safety test and analysis of potency test data, various indexes are stable and effectively; and the use effect of the egg yolk antibody is estimated by a method for injecting after counteracting toxic substances and a method for counteracting toxic substances after injecting. A result shows that the egg yolk antibody of type-2 duck adenovirus infection can effectively prevent and treat the type-2 duck adenovirus infection, and has good commercialization development prospect.

Description

Technical field [0001] The invention belongs to the technical field of veterinary biological products, and specifically relates to a yolk antibody for preventing and treating duck type 2 adenovirus disease and a preparation method thereof. Background technique [0002] Adenovirus is a common infectious pathogen in poultry. Avian adenoviruses are divided into three groups: Group I is avian adenoviruses isolated from respiratory infections of chickens, turkeys, geese and ducks. They have common group-specific antigens and can be divided into A, B, C, D, E5 species and 12 serotypes; Duck type 2 adenovirus belongs to group I avian adenovirus, which was isolated from diseased Muscovy ducks by the French and Hungarians in 1977. Infected ducks mainly showed depression, diarrhea, weight loss and other symptoms. In recent years, the duck industry in my country has developed rapidly, especially in the large number of scattered farmers, some duck raising facilities are rudimentary, the awa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C07K16/02A61K39/42A61P31/20
CPCA61K2039/505A61K2039/552C07K16/02C07K16/081
Inventor 王相芹王丹娜刘阳王学波张海波颜瑞娟胡秀香王赛林文超李朝阳
Owner SHANDONG SINDER TECH
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