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Preparation for restraining adenovirus infection

An adenovirus and preparation technology, applied in the field of preparations for inhibiting adenovirus infection, can solve the problems of epidemic spread, no curative effect, political and economic impact, etc., and achieve the effect of inhibiting infection

Active Publication Date: 2016-05-11
INST OF PLA FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The reality and potential threats of viral infectious diseases are becoming more and more serious for human beings, and the pressure on the prevention and control of sudden infectious diseases is increasing. When the epidemic broke out, the original specific prevention and treatment drugs were difficult to exert their due prevention and control effects, or even had no curative effect, and the limitations of human cognition and scientific research cycle made it difficult for a new generation of specific prevention and treatment drugs with significant curative effects to be introduced Launched in a short period of time, it will easily lead to the rapid spread of the epidemic and catastrophic outbreaks in some areas, which will not only cause great damage to local public health services, but also have a huge impact on society's politics and economy

Method used

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  • Preparation for restraining adenovirus infection
  • Preparation for restraining adenovirus infection
  • Preparation for restraining adenovirus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, preparation of plant extract compound formula adenovirus infection inhibitor

[0039] Epigallocatechin gallate (EGCG), tannic acid and astragalus polysaccharide are uniformly mixed according to a mass ratio of 1:1:1.5 to obtain a plant extract compound formulation.

[0040] Before the experiment, it was dissolved in PBS buffer, made into 2560 μg / ml (the total concentration of EGCG, tannic acid and astragalus polysaccharide in the solution), filtered, and after sterilization, it was stored at -20°C for later use.

Embodiment 2

[0041] Embodiment 2, cytotoxicity test

[0042] In this example, the neutral red phagocytosis method was used to measure the cytotoxicity of the compound formulation prepared in Example 1 to mammalian cells. The specific operations are as follows:

[0043] The compound formulation preparation solution (2560 μg / ml) prepared in Example 1 was diluted step by step to obtain a total of 6 concentration. Then the dilutions of different dilutions were added to the 96-well cell culture plate with 293 cells and the cell density was about 80%, 100 μl per well, and 4 duplicate holes were made for each dilution, and normal cells (that is, untreated Add compound formula preparation) as a control, after 2 hours of action, discard the test solution, add cell maintenance culture medium, add 200 μ l to each well, place it in a cell incubator and cultivate, after 48 hours, add 0.1% (0.1g / 100mL) to each well Neutral red 25μl, after reacting at 37°C for 1.5h, suck out the liquid in each well an...

Embodiment 3

[0048] Embodiment 3, adenovirus infection inhibition test

[0049] In this example, the CPE method was used to measure the inhibitory effect of the compound formulation prepared in Example 1 on adenovirus infection. The tested adenovirus was type 5 adenovirus dl309 strain.

[0050] Take a 96-well culture plate with 293 cells that has grown into a monolayer with a growth density of about 80%, pour off the culture medium, rinse the cells with PBS for 3 times, and add them under the three conditions of A, B, and C respectively. The compound formula preparation that example 1 prepares:

[0051] A. Simultaneously with virus adsorption: put an equal volume of 2×100TCID 50 After mixing the virus solution of type 5 adenovirus with the test substance solution at 2 times the concentration, add 100 μl / well of the mixed solution to the cell culture plate, place it in the cell culture incubator, and discard it after the virus is adsorbed for 1 hour. After washing the cell surface 3 time...

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Abstract

The invention discloses a preparation for restraining adenovirus infection. The preparation is applied to 1 preparing a product for restraining adenovirus infection and 2 restraining adenovirus infection. The preparation is mainly formed by mixing epigallocatechin gallate, tannin and astragalus polysaccharide. The mass ratio of epigallocatechin gallate to tannin to astragalus polysaccharide is (0.5-1.0):(0.5-1.0):(0.5-1.5). The cytotoxicity of the complex formula preparation is not higher than that of a control sample ribavirin obtaining security permission, and the preparation is safe. Under safe use concentration, type 5 adenovirus infection can be effectively restrained through preventive use of the preparation, and the preparation has commercial value for being further developed into an adenovirus infection inhibitor.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a preparation for inhibiting adenovirus infection. Background technique [0002] Acute infectious disease caused by adenovirus, easily invades respiratory tract and digestive tract mucosa, conjunctiva, urinary tract and lymph nodes. The main manifestation is acute upper respiratory tract infection (acute respiratory tract infection caused by adenovirus accounts for 2% to 4%), followed by eye and gastrointestinal tract infection. The crowd is generally susceptible, more common in children. About half of the patients were asymptomatic infection. Infants and young children are susceptible to adenovirus pneumonia, which is severe and has a high mortality rate. No specific treatment. According to the population serum-specific antibody survey and virus isolation, it can be known that adenovirus infection is very widespread. The source of infection is patients and latent infected persons....

Claims

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Application Information

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IPC IPC(8): A61K36/481A61K31/715A61P31/20A61K31/353A61K31/7024
Inventor 常国辉刘京梅杨益黄留玉罗丽晓李瑾惠罗彦军孙走南唐玥苏文莉张洁刘璇
Owner INST OF PLA FOR DISEASE CONTROL & PREVENTION
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