Resistance evaluation method for potato late blight

A technology for potato late blight and potato late blight, applied in the field of bacterial wilt resistance identification, can solve the problems of irregularities, limitations, and inconsistent evaluation methods of disease resistance, and achieve high repeatability and strong stability

Pending Publication Date: 2022-07-22
湖北恩施中国南方马铃薯研究中心
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Problems solved by technology

At the same time, in actual production, it is found that different varieties have different levels of resistance to late blight, and the disease resistance of varieties also changes with the passage of planting years. There is a lack of scientific operating specifications on how to carry out identification of disease resistance of varieties In this regard, combined with the epidemic characteristics of potato late blight, continuous follow-up investigations have been carried out to form a practical and effective resistance evaluation method
[0004] The occurrence and prevalence of potato late blight are closely related t

Method used

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  • Resistance evaluation method for potato late blight
  • Resistance evaluation method for potato late blight
  • Resistance evaluation method for potato late blight

Examples

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Embodiment 1

[0051] In Yangtianping Village, Sancha Town, Enshi City, a field with convenient transportation, flat terrain, uniform fertility, and convenient irrigation and drainage was selected. The potato varieties tested were designed in random blocks, repeated three times, and each plot was planted with one ridge (double row), There are 15 plants in each row, and a total of 90 plants are planted in each variety. The test varieties include the high-susceptibility variety "Hisen No. 6". The aisle between the plots is 50 cm, and 5 rows of early-maturing induced varieties (“Feu Ruita”) and mid-to-late-maturing induced varieties (“Mira”) were planted around the test four weeks respectively. 2 +Organic fertilizer 100kg / 667m 2 As a base fertilizer, mechanical ridges are uniformly covered with film, and the film is punched at a fixed point, and the whole potato is sown on January 30, 2020. An artificial spray system is set up between the potato ridges. The effluent is a fine mist. The spacing...

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Abstract

The invention relates to a potato late blight resistance evaluation method. The method comprises the following steps: 1) identifying physiological races of potato late blight bacteria; (2) inoculating a potato late blight bacterium spore suspension; 3) investigating potato late blight, wherein the morbidity is equal to diseased plant number/total plant number * 100%; 4) establishing a potato late blight nursery; and 5) evaluating the field resistance of the potato late blight: evaluating the disease resistance on the basis of the area AUDPC under a disease development curve, expressing the AUDPC value by using the percentage value of the potato susceptibility rate in a period of time, converting the corrected relative AUDPC value CrAUDPC from the AUDPC, and judging the infection type by using the CrAUDPC value as an evaluation basis according to a formula. According to the method, a systematic and scientific potato late blight field resistance evaluation method is formed, and the identification result has comparability, reliability and stability.

Description

technical field [0001] The invention relates to the technical field of bacterial wilt resistance identification, in particular to a resistance evaluation method of potato late blight. Background technique [0002] Potato is the fourth largest food crop in my country. Potato late blight caused by Phytophthora infestans is one of the important bottlenecks restricting the development of my country's potato industry. For a long time, the breeding and utilization of disease-resistant varieties has been the most economical and effective strategy to control late blight. The breeding and promotion of potato disease-resistant varieties are actively carried out in China, which have broad application prospects in production. In the process of disease resistance breeding, it is usually necessary to establish a stable and accurate identification method for disease resistance, so that the breeding materials for disease resistance can be accurately selected. Establishing a disease nurser...

Claims

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Application Information

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IPC IPC(8): C12Q1/18A01G7/06A01G22/25
CPCC12Q1/18A01G7/06A01G22/25G01N2333/37
Inventor 肖春芳王甄沈艳芬张等宏闫雷邹莹高剑华杨国才宋威武
Owner 湖北恩施中国南方马铃薯研究中心
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