Real-time quantitative analysis method and instrument for piezoelectric gene diagnosis

Abstract

The present invention adopts piezoelectric gene diagnosis chip and frequency detection instrument and utilizes multiple PCR technique to make temp. circulation of hydridization-extension-denaturation to amplify, target gene, monitor and obtain the linear relationship of frequency change and target gene concentration, then uses external standard curve to make quantitative analysis. Said apparatus comprises shell body, power supply and its connected circuit, and main body module, analysis, software, sample preparation module, positioning liquid flow module and piezoelectric gene diagnosis chip detection module. Said invention is suitable for clinical diagnosis and molecular mechanism research, monitoring epidemic disease and infections disease spreading and harmful microbial genesis and diffusion.

Description

technical field [0001] The invention belongs to an in vitro gene detection method and an instrument for implementing the method. Background technique [0002] The occurrence and development of most diseases are related to the patient's genetic background or its changes. Genetic diagnosis refers to the method of using genetic analysis to diagnose diseases. The object of genetic diagnosis is the invasion of pathogenic organisms first. Direct detection of the genetic material of pathogenic organisms can greatly improve the sensitivity and specificity of diagnosis, and when commercial antibodies cannot be obtained, genetic diagnosis becomes the detection of pathogenic microbial infections, especially viruses. The only means of infection; followed by congenital genetic diseases, some genetic diseases whose etiology is determined and other diseases whose etiology is not yet clear may be related to the possession or change of one or some genes; the third is acquired gene mutations ...

AI Technical Summary

Problems solved by technology

[0007] The above-mentioned real-time fluorescence quantitative PCR and gene chip technologies, on the one hand, all adopt fluorescence detection, which requires large-scale, complex, sophisticated and expensive detection equipment, and takes a long time (3-4 hours), that is, it needs to be tested multiple times (more than 15 times) ) after the amplification cycle, or after the hybridization reaction is completed, and there are many factors that affect the accuracy of the analysis results, it is difficult to control and grasp, and it is easy to produce false positive results, and the target gene or probe needs to be fluorescently labeled, which increases the consumption. Reagent costs; on the other hand, an analysis process includes multi-step operations, especially including sample pretreatment, target gene acquisition and labeling, etc., the operation is cumbersome, and the entire analysis system is difficult to integrate, automate and miniaturize

These defects are insurmountable obstacles to the popularization of genetic diagnosis technology at present

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