Monoclonal antibody CAb-1 heavy and light chain variable region gene of human carcinoma of large intestine, and its uses

A technology of monoclonal antibody and variable region, which is applied in the fields of application, antibody, gene therapy, etc., and can solve problems such as difficult to obtain high-affinity and high-specificity colorectal cancer monoclonal antibody

Active Publication Date: 2004-11-17
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
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Problems solved by technology

Due to the mutation or partial loss of tumor antigens, it is difficult to obtain high-affinity and high-specificity colorectal cancer monoclonal antibodies by this method

Method used

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  • Monoclonal antibody CAb-1 heavy and light chain variable region gene of human carcinoma of large intestine, and its uses
  • Monoclonal antibody CAb-1 heavy and light chain variable region gene of human carcinoma of large intestine, and its uses
  • Monoclonal antibody CAb-1 heavy and light chain variable region gene of human carcinoma of large intestine, and its uses

Examples

Experimental program
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Embodiment Construction

[0015] (1) Cloning of light and heavy chain variable region genes of anti-human colorectal cancer monoclonal antibody CAb-1

[0016] The cell line used is a high-affinity, high-specificity mouse-derived anti-human colorectal cancer monoclonal antibody CAb-1 hybridization obtained by the inventors by using fresh colorectal cancer tissue homogenate to immunize mice and using traditional fusion methods tumor cell lines. The secreted antibody does not bind to tumor-associated antigens such as CEA, 17-1A, TAG-72, CEA, A33, etc., and is aimed at a new tumor-associated glycoprotein antigen with a molecular weight of about 200KD. Its secreted antibody molecular subtype is IgG1κ.

[0017] CAb-1 hybridoma cells (5×10 6 ), using guanidine isothiocyanate one-step method to extract total RNA, and take a small amount for quantification by UV spectrophotometer and detection by 1% formaldehyde-denaturing agarose gel electrophoresis. Then Oligo(dT) 15 (Promega Company) was used as a random...

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Abstract

The invention relates to monoclonal antibody CAb--1 heavy and light chain variable region gene of human carcinoma of large intestine, the encoded polypeptides by the said genes, and the use of the genes and the polypeptides in preparing the medicament for diagnosis or treatment of colon tumor or rectum tumor, wherein a set of designed expanded primers are introduced. Based on the gene, gene engineering method can be employed to construct and express small molecular genetic engineering antibody of various forms, such as Fab antibody, chimeric antibody, and antibody fusion protein, which can be used in preparing medicament for diagnosis or treatment of large intestine (colon, rectum) cancers.

Description

technical field [0001] The present invention relates to the light and heavy chain variable region genes of an anti-human colorectal cancer monoclonal antibody CAb-1 and the encoded polypeptides thereof, and the application of the genes and polypeptides in the preparation of medicaments for diagnosing and treating colorectal cancer . Background technique [0002] Colorectal cancer is a common malignant tumor that endangers the population. Its incidence rate ranks second in Europe and the United States, and ranks third in my country. With the continuous adjustment of people's diet structure, its incidence rate still has an upward trend. Therefore, it is of great significance to study the diagnosis and treatment of colorectal cancer. Although the current nuclear magnetic resonance and endoscopic technology continue to develop, the detection rate of early colorectal cancer tumors has increased, but there are still false positives and false negatives in clinical actual detectio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7088A61K39/395A61K48/00A61P35/00C07H21/04C12N15/12C12P19/34C12Q1/68G01N33/577
Inventor 陈志南邢金良杨向民张思河
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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