Seed selection and hybridized variety production method of sabbage type rape micropowder less cytoplasam male sterile series

A cabbage rape, male sterile line technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of leaf yellowing, lack of restorer lines, nectary dysplasia and the like

Inactive Publication Date: 2004-12-22
NORTHWEST A & F UNIV
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Problems solved by technology

Ogu CMS is a natural male sterile plant discovered by Ogura in Kagoshima, Japan in 1968 from a radish population. After this sterile material was transferred to Brassica napus, the fertility of t

Method used

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Embodiment Construction

[0020] The present invention will be described in further detail below in conjunction with specific examples.

[0021] 1. Breeding of CMS lines:

[0022] Use ledos (Germany, double low) as the female parent and Zhongshuang 2 (China, double low) as the male parent for crossing. Five male sterile plants were found in the progeny population of this combination. The petals of this sterile material are small, and the stamens are brown, similar to nuclear sterility. Obviously, but the flowers that bloom in the later period are very sterile, and the artificial buds are self-fertilized without fruiting. The sterility was maintained with materials from different sources, and the results showed that the maintainer lines of Polima and Shaanxi 2A CMS could well maintain the sterility of 212A, and all the transgenic CMS lines had no trace pollen production, and These sterile lines all had the phenomenon of dead buds at the initial flowering stage. The CMS line 212A with strong growth po...

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Abstract

A method for selectively breeding the crossed cabbage-type rape includes such steps as crossing between European rape ledos and Chinese rape Shuang No.2 to obtain sterile line, back crossing with Chinese rape Shuang No.2 to obtain non-micropollen sterile line and relative maintenance line, copmlex crossing to obtain restoring line, and diallel crossing to obtain Shanyou No.6 and No.8, which feature high yield.

Description

technical field [0001] The invention belongs to agricultural rapeseed heterosis utilization technology, and relates to a method for selecting and breeding a non-micronized type cytoplasmic male sterile line of Brassica napus and producing a hybrid. Background technique [0002] There are many ways to utilize heterosis in rapeseed, mainly including cytoplasmic sterile hybrids, nuclear sterile hybrids, chemical killing hybrids and self-incompatibility hybrids. At present, three cytoplasmic sterile lines (ie, sterile lines, maintainer lines, and restorer lines) are mainly used to produce hybrids. The cytoplasmic male sterile lines of rape found at home and abroad mainly include Polima CMS, Shan 2A, Ogu CMS, nap CMS, nig CMS, mur CMS, MI CMS, Nca, and RM5637. Polima CMS is a natural male sterile strain discovered by Professor Fu Tingdong in Polish Brassica napus Polima in 1972. According to its performance, it can be divided into high-temperature sterile type, low-temperature s...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 董振生刘创社董军刚刘绚霞李红兵
Owner NORTHWEST A & F UNIV
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