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Polysaccharide of basidium and its use

A kind of technology of polysaccharide and fungi, applied in the field of polysaccharide of Antrodia camphorata basidiomycetes

Inactive Publication Date: 2005-05-25
陈介甫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although in Taiwan folks, Antrodia camphorata has been widely used to treat liver diseases and cancers, especially viral hepatitis, so far there are very few reports on the establishment of Antrodia camphorata strains, genetic research, culture conditions, and quality control.

Method used

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  • Polysaccharide of basidium and its use
  • Polysaccharide of basidium and its use
  • Polysaccharide of basidium and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] liquid culture

[0050] Antrodia camphorata bacterial strains were placed in slanted potato dextrose agarslant medium (PDA, potato dextrose agarslant), replaced once in three weeks, and the strains were inoculated in the center of the sterilized 25ml potato mashed agarslant medium (PDA) culture dish. Cultivate at 28°C for 19 days, cut the successfully cultured mycelia into 1×1cm, place them in 25ml of mashed potato and agar medium (PDA), add 20g / L of glucose (glucose), and fill with pH=5.6, After culturing in the dark for 26 days in a 250ml bottle, wash the cells with 250mM sodium chloride (NaCI), centrifuge at 3000 rpm to remove extracellular polysaccharides and culture medium, freeze-dry, and store in a refrigerator at 4°C.

Embodiment 2

[0052] Polysaccharide composition analysis

[0053] Antrodia camphorata (CCRC35396, CCRC35398, B71, B85, B86) strains (CCRC35396, CCRC35398, B71, B85, B86) were placed in slanted potato mashed agarslant medium (PDA, potato dextrose agarslant, purchased from Sigma, USA, containing potato extract, glucose and agarslant). ) culture, the mycelia were preserved in the mashed potato liquid medium (PDB) culture medium and placed in the dark for 26 days. After washing, centrifuging, and freeze-drying the mycelia. After extracting with 1:100 (w / w) 80°C water, add 4 times the volume of 95% alcohol, precipitate in a refrigerator at 4°C overnight, then centrifuge to collect the precipitate, and freeze-dry to obtain the crude polysaccharide. The crude polysaccharides were placed on an anion exchange resin, analyzed by a mixed solvent of sodium acetate (NaOAc) and 90mM sodium hydroxide at room temperature, and finally collected data with PRIMEDAK software, the result...

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Abstract

A polyose of antrodia cinnamonea basidiomycetes is prepared from the strains CCRC 35396, CCRC 35398, B71, B85 and B86 of antrodia cinnamonea, and can be used to prevent the treat viral hepatitis B.

Description

technical field [0001] The invention relates to a polysaccharide of antrodia antrodia basidiomycete and its medical application. Background technique [0002] Antrodia camphorata (Antrodia camphorata) is a fungus endemic to Taiwan, which grows on the unique camphor tree (Cinnamomum kanehirae) in Taiwan. Antrodia Cinnamomea is a new species of Basidiomycotina, Fungi, Anamorphales, Polyporaceae, Antrodia genus. [0003] Because fungi have the ability to produce secondary metabolites, and produce a variety of secondary metabolites, based on the ability of fungi to be easily induced, it is not affected by regional geography and seasonal environment. Therefore, fungi such as Cordyceps sinensis, Ganoderma lucidum, and Poria cocos can be produced in large quantities by fermentation, and new drugs can be developed. [0004] Antrodia camphorata has been used as a folk drug for treating tumor diseases in Taiwan. Its chemical composition includes glycosides, steroids and triterpenes....

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P1/16C08L37/00
Inventor 陈介甫卢美光黄瑞龄张东柱陈其婷王世任
Owner 陈介甫
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