Chinese herbal medicinal diluent for cow frozen semen

A technology of freezing semen and Chinese herbal medicine extracts, which is applied in the fields of application, preservation of human or animal bodies, and medical raw materials derived from mammals, etc., to achieve the effect of solving residual side effects

Inactive Publication Date: 2006-02-08
NORTHWEST A & F UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

However, there are very few reports on the effect of adding Chinese herbal medicine extracts directly to the semen dil

Method used

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Embodiment Construction

[0055] The realization of the present invention can be applied in dairy cow breeding centers, livestock improvement stations in various places, or livestock artificial insemination centers, etc. After purchasing Chinese herbal medicines according to the dosage required by the formula, first extract their active ingredients, filter and sterilize them, and replace them when making frozen semen Part of the base fluid (the main components of the base fluid: glucose 15g / L, lactose 30g / L, glycine 8g / L), and then make frozen semen according to the corresponding freezing method.

[0056] Formula: base liquid, containing Chinese herbal medicines Epimedium, Morinda officinalis, Dodder and licorice; the weight ratio formula of herbs is: Epimedium 35-37%, Morinda officinalis 27-30%, Dodder 20-22%, Licorice 12-15%, the sum of Chinese herbal medicine raw materials is 100%.

[0057] When extracting Chinese herbal medicines, first accurately weigh the different Chinese herbal medicines in the...

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Abstract

The invention discloses a Chinese herbal medicinal diluent for cow frozen semen, which comprises basic solution and extract of Chinese medicinal herbs, the content of the Chinese medicinal herb extract is 10-14% by volume ratio of the basic solution, 1L of the basic solution contains glucose 15g, lactin 30g, and glycine 8g. The Chinese medicinal herb extract is prepared from epimeddium, morinda root, dodder seed and and licorice root.

Description

technical field [0001] The invention relates to a diluent of frozen semen in the improvement of livestock artificial insemination technology, in particular to a diluent of Chinese herbal medicine for bovine frozen semen. Background technique [0002] In 1780, the Italian zoologist Spallanznni put dog semen into snow for freezing, and observed the activity of sperm after thawing at room temperature, thus starting the research on the preservation of semen below 0°C. At the beginning of the 20th century, the research on semen freezing with refrigeration technology or artificial solid gas began to appear, and gradually attracted the attention of scientists from all over the world. In 1908, Maximov immersed plant tissues and cells in glycerol solution and cooled the temperature to -22°C. It was found that some cells survived. This was the first report of using a cryoprotectant. In 1937, Bernslain et al. used 9.2% (1M) glycerol solution to successfully pre...

Claims

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Application Information

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IPC IPC(8): A01N1/00A01N65/00A61K35/12
Inventor 李青旺胡建宏昝林森刘永峰
Owner NORTHWEST A & F UNIV
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