Method for detecting tubercle bacillus gene

A technology for detecting Mycobacterium tuberculosis, which is applied in the detection field of Mycobacterium tuberculosis gene, can solve the problems of expensive equipment and difficult popularization and use, and achieve the effects of high accuracy, intuitive diagnosis results and overcoming false positives

Inactive Publication Date: 2006-03-08
GUANGXI MEDICAL UNIVERSITY
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Problems solved by technology

The biggest advantage of this method is that it is efficient and accurate, but the equipment is expensive and it is difficult to popularize and use it.
[0007] At present, the Ministry of Health requires that the detection rate of Mycobacterium tuberculosis in sputum samples of tuberculosis patients in various places should reach 60%. The rate is also lower than 30%, which is still far from the national requirement

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  • Method for detecting tubercle bacillus gene

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Embodiment Construction

[0022] The following briefly describes the main principles, equipment, reagents and specific steps of the Mycobacterium tuberculosis gene detection method:

[0023] 1. Principle: First, use a pair of designed specific primers P1 and P2 to amplify the sample by PCR. The amplified target gene is a 245bp region in the unique insertion sequence IS6110 of Mycobacterium tuberculosis. The IS6110 insertion sequence is Mycobacterium tuberculosis A multi-copy conserved fragment in the genome, with a total sequence length of about 1300bp, is mainly found in human Mycobacterium tuberculosis with IS6110. The 5' end of primer P1 was labeled with biotin, and a large number of DNA products containing P1 primer and extension part were amplified after PCR reaction, and these products all contained biotin. Then the T1 probe, T2 probe and T3 probe specifically hybridize with the corresponding sequence of the extended part in the product respectively. After hybridization, the target gene with bio...

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Abstract

This invention relates to a test method for tubercle bacillus genes, which uses a pair of specific primers P1 and P2 to carry out PCR amplification to a region of 245 bp in a plug-in sequence IS6110 specially owned by the tubercle bacillus, in which, 5' end of P1 has biotin and carries out specific cross to the PCR product and the probe on a film chip then enzyme-link reaction, finally to express the judgment result by display colors.

Description

technical field [0001] The invention belongs to a detection method for tubercle bacillus gene. Background technique [0002] my country is one of the countries with severe tuberculosis infection. According to the data released recently by the Ministry of Health of my country, the number of tuberculosis patients in the country has exceeded 100 million, and the total number of existing tuberculosis patients is close to 5 million, of which infectious patients account for about half. Each infectious patient can infect 15-20 people per year. Once the disease develops into drug-resistant tuberculosis, it is difficult to cure and the case fatality rate is extremely high. Therefore, tuberculosis is one of the infectious diseases that seriously endanger people's health. my country attaches great importance to the prevention and treatment of tuberculosis, and requires the country to detect tuberculosis patients as efficiently as possible so that they ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 何敏周凌云何晓
Owner GUANGXI MEDICAL UNIVERSITY
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