Method for extracting bilobalide B from ginkgo leaf or ginkgo leaf extract

A technology of ginkgolide and ginkgo biloba, applied in the direction of organic chemistry, etc., can solve the problems of low yield, time-consuming, cumbersome operation, etc.

Inactive Publication Date: 2006-10-11
孙毅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the extraction of ginkgolide B mostly adopts column separation and microwave extraction at home and abroad, and the operation is cumbersome, time-consuming, and the yield is not high; while the method of the present invention is simple and easy, low in cost, high in yield, and has no resin residue. The purity of the obtained ginkgolide B is more than 95%, and the yield is more than 1%.

Method used

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Examples

Experimental program
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Embodiment Construction

[0011] (1) Take ginkgo biloba extract, add 3 times the amount of water, boil, refrigerate overnight, take the supernatant, filter, and collect the filtrate.

[0012] (2) Add n-butanol solution 5 times the volume of the filtrate to extract once, separate the water layer, add n-butanol solution 3 times the volume of the water to extract four times, and combine the extracts.

[0013] (3) The extract was recovered under reduced pressure at 60°C for the n-butanol solution, evaporated to dryness, and the dry matter was collected;

[0014] (4) Add 40% ethanol solution 5 times the weight of the dry matter, boil to dissolve, refrigerate overnight, filter with suction, and wash with a small amount of 40% ethanol solution to obtain crystals;

[0015] (5) Add 500 ml of absolute ethanol to the above crystals, heat to dissolve, refrigerate overnight, filter with suction, and take ginkgolide B crystals; repeat 3 times.

[0016] (6) Ginkgolide B crystals were dried under vacuum at 60C for 5 ...

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PUM

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Abstract

The invention discloses a novel process for extracting bilobalide B from ginkgo leaf or ginkgo leaf extract through liquid-liquid extraction and refining, wherein the extraction solvent being n-butanol or other solvents having similar dissolving property and polarity. The obtained bilobalide B has a purity higher than 95% and a yield higher than 1%.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine preparation, and relates to a new method for extracting active ingredients of medicine from ginkgo leaves or ginkgo leaf extracts. Background technique [0002] Ginkgo biloba (Ginkgo hiloba L.) is a plant of the genus Ginkgo in the Ginkgo family. The ginkgo tree appeared 200 million years ago and was nearly extinct during the Ice Age. It only survived in my country and was called "living fossil" by Darwin. With the deepening of the research on Ginkgo biloba at home and abroad, it was found that ginkgolide B, the active ingredient in Ginkgo biloba, is a highly effective platelet activating factor antagonist, which has unique pharmacological effects and therapeutic value, which has aroused great interest in the pharmaceutical industry worldwide. big interest. [0003] The sources of ginkgolide B can be divided into artificial cultivation, chemical synthesis and plant cell cult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/22C07D307/00
Inventor 孙毅高红王晓玲张健
Owner 孙毅
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