Pupa cordyceps fruiting body cultured using large galleria waxmoth larva and its culturing method
A cultivation method, the technology of the wax moth, which is applied in the direction of fungi, etc., can solve the problems that there is no use of the larvae of the wax moth to cultivate the fruiting bodies of Cordyceps
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Embodiment 1
[0024] With potato 20% (mass fraction, the same below), glucose 2%, KH 2 PO 4 0.3%, MgSO 4 .7H 2 O0.15%, Vitamin B 1 20mg / L, 2% agar, and 0.5% of the larvae of Mellonella mellonella were prepared as a primary medium, with 2% glucose, 0.4% peptone, and KH 2 PO 4 0.4%, MgSO 4 .7H 2 O 0.4%, Vitamin B 1 20mg / L, 0.5% of the larvae of Mellonella mellonella, pH 6.5 to prepare the secondary medium.
[0025] Inoculate the isolated and purified Cordyceps militaris strains into the primary culture medium on a solid slant, incubate at 20°C in the dark for 7 days, until the white fluffy hyphae cover the slope, and then connect the primary strains from the plate to the sterilized In the liquid culture medium of the second-grade bacterial species, shake culture at 25°C and 120r / min for 7 days until the bacterial balls are filled with the liquid culture medium. The larvae of Mellonella mellonella were sterilized with ethanol with a mass fraction of 75%, and then 0.01 mL of bacteria ...
Embodiment 2
[0028] With potato 20%, glucose 2%, KH 2 PO 4 0.3%, MgSO 4 .7H 2 O0.15%, Vitamin B 1 20mg / L, 2% agar, and 5.0% of the larvae of Mellonella mellonella were used to prepare the primary medium, with 2% glucose, 0.4% peptone, and KH 2 PO 4 0.4%, MgSO 4 .7H 2 O0.4%, Vitamin B 1 20mg / L, 5.0% of the larvae of Mellonella mellonella, pH 6.5 to prepare the secondary medium.
[0029] Inoculate the isolated and purified strains of Cordyceps militaris to the primary culture medium on a solid slant, and culture them in the dark at 25°C for 10 days until the white fluffy hyphae cover the slope, and then connect the primary strains from the plate to the sterilized In the liquid culture medium of the secondary strains, shake culture at 25°C and 120r / min for 10 days, until the balls are filled with the liquid medium, and then inject 0.2mL of the strains into the larvae of the artificially cleaned and reared Great Melonella moth with a sterile syringe .
[0030] Place the infected la...
Embodiment 3
[0032] With potato 20%, glucose 2%, KH 2 PO 4 0.3%, MgSO 4 .7H 2 O0.15%, Vitamin B 1 20mg / L, 2% agar, 3.0% substance of Mellonella mellonella larvae to prepare primary medium, with 2% glucose, 0.4% peptone, KH 2 PO 4 0.4%, MgSO 4 .7H 2 O0.4%, Vitamin B 1 20mg / L, 3.0% of the larvae of Mellonella mellonella, pH 6.5 to prepare the secondary medium.
[0033] Inoculate the isolated and purified Cordyceps militaris strains into the primary culture medium on a solid slant, and incubate for 8 days in the dark at 23°C until the white fluffy hyphae cover the slope, and then connect the primary strains from the plate to the sterilized In the liquid culture medium of the second-grade bacterial species, shake culture at 25°C and 120r / min for 8 days until the bacterial balls are filled with the liquid culture medium. The surface of the larvae of Mellonella mellonella was disinfected with ethanol with a mass fraction of 75%, and then the larvae of Mellonella mellonella were soaked w...
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