PML-RARa gene fluorescence quantitative RT-PCR primer and probe and reagent kit

Abstract

The invention discloses a quantitative RT-PCR primer and probe and agent box of PML-RARa fusing gene mRNA fluorescence with BCR-ABL fusing gene primer and probe sequence as SEQ ID NO1-4 and internal reference gene primer and probe sequence as SEQ ID NO5-7, wherein the agent box contains cell cracking liquid, water, RT-PCR reacting liquid, internal reference G6PDHRT-PCR reacting liquid, BCR-ABL fusing gene detecting probe, G6PDH internal reference gene testing probe, composite enzyme, standard material and comparing material; the invention measures the expressive level of L-pattern, S-pattern and V-pattern mRNA simultaneously, rapidly and precisely, which improves inducing buffer rate and lessens early bleeding death.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to quantitative reverse transcription PCR detection of gene mRNA expression. Background technique [0002] The main feature of acute promyelocytic leukemia (APL) is t(15;17)(q22,q11) chromosome translocation. Due to the difference in the position of the PML breakpoint on chromosome 15, the fusion protein formed is also different. The fusion gene formed at the breakpoint of PML bcr3 encodes a short form of PML-RARa protein, while the fusion gene formed at the breakpoint at PML bcr1 can produce two fusion proteins; the long form and the intermediate form of PML-RARa. The intermediate PML-RARa removes exon 5 of PML during mRNA splicing. The long form and the intermediate form are often co-expressed in the same patient. Compared with the long form, the short form lacks a proline and serine-rich region of 158 amino acids in length, and this region is a potential phosphorylation site...

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by this invention is to provide a kind of PML-RARa fusion gene mRNA fluorescent quantitative RT-PCR primer and probe and test kit, to overcome prior art PML / RARa in acute promyelocytic leukemia (APL) cell The quantitative detection of fusion gene mRNA expression level has poor accuracy, low sensitivity, and slow detection speed, which is not conducive to timely treatment according to the expression of PML / RARa fusion gene

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