Diagnosis and treatment of cancer

a cancer and cancer technology, applied in the field of cancer diagnosis and treatment, can solve the problems of inability to fit the sigmoid curve to the data, two extremes of this curve were unknown or undetermined, and the pharmacological properties of the vgs current in the rat mat-lylu cell were not consistent with those reported

a cancer and cancer technology, applied in the field of cancer diagnosis and treatment, can solve the problems of inability to fit the sigmoid curve to the data, two extremes of this curve were unknown or undetermined, and the pharmacological properties of the vgs current in the rat mat-lylu cell were not consistent with those reported

US20060166194A1Inactive Publication Date: 2006-07-27IMPERIAL INNOVATIONS LTD
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  • Diagnosis and treatment of cancer
  • Diagnosis and treatment of cancer
  • Diagnosis and treatment of cancer

Examples

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example 3

Voltage-Gated Na+ Channel Expression Correlates with Pathological Progression in Human Prostate Cancer

[0379] Previous electrophysiological studies have shown that functional voltage gated Na+ channels (VGSCs) are expressed selectively by highly metastatic cell lines of rat (MAT-LyLu) and human (PC-3) prostate cancer. Furthermore, blockage of these channels with tetrodotoxin significantly reduced the cells' invasion in vitro (Grimes et al. (1995) FEBS Letts. 369, 290-294; Laniado et al (1997) Am. J. Pathol. 150 1213-1221. Direct, positive correlation between invasiveness and Na+ channel protein expression by several different rat and human prostate cancer cell lines was subsequently demonstrated by Smith et al. (1998) FEBS Lens. 423,19-24. However, it is not known whether VGSC expression occurs in human prostate cancer in vivo, and if so, whether the levels of expression relate to the metastatic profile of the constituent malignant epithelial cells.

[0380] Immunohistochemical locali...

example 4

Regulation of VGSC Function in Prostate Cancer Cell Lines

[0382] VGSC functional expression in rodent MAT-LyLu cells was investigated. Epidermal growth factor (EGF) upregulates VGSC functional expression. As shown in FIG. 9, exogenous EGF potentiates the functional VGSC expression. This effect is blocked by co-application of EGF receptor kinase inhibitor (AG1478; Calbiochem, San Diego, Calif., USA). Application of EGF receptor antibody (Oncogene, Cambridge, Mass., USA) also reduces the basal VGSC current implying that the phenomenon occurs endogenously.

[0383] Other growth factors (for example nerve growth factor) and hormones (including androgens) may have similar effects.

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Abstract

A method of diagnosing cancer in a human patient comprising the steps of (i) obtaining a sample containing nucleic acid and / or protein from the patient; and (ii) determining whether the sample contains a level of hNeNa voltage-gated Na+ channel nucleic acid or protein associated with cancer. A method of treating cancer comprising the step of administering to the patient an agent which selectively prevents the function of hNe—Na voltage-gated Na+ channel. A genetic construct comprising a nucleic acid encoding a molecule capable of preventing the function of hNeNa voltage-gated Na+ channel expressed in a cell. The methods and compositions are particularly suited to prostate cancer.

Description

[0001] The present invention relates to methods of determining whether a patient has cancer and whether the cancer is likely to metastasise; and it relates to methods of treating cancer, particularly prostate cancer. [0002] Cancer is a serious disease and a major killer. Although there have been advances in the diagnosis and treatment of certain cancers in recent years, there is still a need for improvements in diagnosis and treatment. [0003] Cancer is a genetic disease and in most cases involves mutations in one or more genes. There are believed to be around 60,000 genes in the human genome but only a handful of these genes have been shown to be involved in cancer. Although it is surmised that many more genes than have been presently identified will be found to be involved in cancer, progress in this area has remained slow despite the availability of molecular analytical techniques. This may be due to the varied structure and function of genes which have been identified to date whi...

Claims

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Application Information

Patent Timeline
27 Jul 2006
Publication
US20060166194A1
IPC
C12Q1/68; G01N33/574; G01N33/50; A61K31/7115; A61K38/00; A61K38/43; A61K45/00; A61K48/00; A61P35/00; C07K14/47; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12N15/113; C12Q1/6886; G01N33/15; G01N33/566
CPC
A61K38/00; C12N15/113; C12Q1/6886; C12Q2600/158; G01N33/574; C12Q2600/136; A61P35/00
Inventors
ALI DJAMGOZ, MUSTAFA; DISS, JAMES