Treatment of hepatocyte growth factor (HGF) related diseases by inhibition of natural antisense transcript to hgf

a technology of hepatocyte growth factor and antisense transcript, which is applied in the direction of antibacterial agents, animal cells, dna/rna fragmentation, etc., can solve the problems of interfering with rna splicing, transcription, translation, replication, etc., and achieve the effect of modulating function and/or expression

Active Publication Date: 2013-07-18
CURNA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0008]Another embodiment provides a method of modulating function and / or expression of an HGF polynucleotide in patient cells or tissues in vivo or in vitro comprising contacting said cells or tissues with an antisense oligonucleotide 5 to 30 nucleotides in length wherein said oligonucleotide has at least 50% sequence identity to a reverse complement of the an antisense of the HGF polynucleotide; thereby modulating function and / or expression of the HGF polynucleotide inpatient cells or tissues in vivo or in vitro.
[0009]Another embodiment provides a method of modulating function and / or expression of an HGF polynucleotide in patient cells or tissues in vivo or in vitro comprising contacting said cells or tissues with an antisense oligonucleotide 5 to 30 nucleotides in length wherein said oligonucleotide has at least 50% sequence identity to an antisense oligonucleotide to an HGF antisense polynucleotide; thereby modulating function and / or expression of the HGF polynucleotide in patient cells or tissues in vivo or in vitro.

Problems solved by technology

Antisense nucleotides, for example, disrupt gene expression by hybridizing to target RNA, thereby interfering with RNA splicing, transcription, translation, and replication.

Method used

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  • Treatment of hepatocyte growth factor (HGF) related diseases by inhibition of natural antisense transcript to hgf

Examples

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example 1

Design of Antisense Oligonucleotides Specific For a Nucleic Acid Molecule Antisense to a Hepatocyte Growth Factor (HGF) and / or a Sense Strand of HGF Polynucleotide

[0231]As indicated above the term “oligonucleotide specific for” or “oligonucleotide targets” refers to an oligonucleotide having a sequence (i) capable of forming a stable complex with a portion of the targeted gene, or (ii) capable of forming a stable duplex with a portion of an mRNA transcript of the targeted gene.

[0232]Selection of appropriate oligonucleotides is facilitated by using computer programs that automatically align nucleic acid sequences and indicate regions of identity or homology. Such programs are used to compare nucleic acid sequences obtained, for example, by searching databases such as GenBank or by sequencing PCR products. Comparison of nucleic acid sequences from a range of species allows the selection of nucleic acid sequences that display an appropriate degree of identity between species. In the ca...

example 2

Modulation of HGF Polynucleotides

Treatment of CHP212 Cells With Antisense Oligonucleotides

[0240]CHP212 cells from ATCC (cat# CRL-2273) were grown in growth media (MEM / F12 (ATCC cat # 30-2003 and Mediatech cat# 10-080-CV) +10% FBS (Mediatech cat# MT35-011-CV)+ penicillin / streptomycin (Mediatech cat# MT30-002-C1)) at 37oC and 5% CO2. One day before the experiment the cells were replated at the density of 1.5×105 / ml into 6 well plates and incubated at 37oC and 5% CO2. On the day of the experiment the media in the 6 well plates was changed to fresh growth media. All antisense oligonucleotides were diluted to the concentration of 20 μM. Two μl of this solution was incubated with 400 μl of Opti-MEM media (Gibco cat#31985-070) and 4 μl of Lipofectamine 2000 (Invitrogen cat# 11668019) at room temperature for 20 min and applied to each well of the 6 well plates with CHP212 cells. Similar mixture including 2 μl of water instead of the oligonucleotide solution was used for the mock-transfected...

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Abstract

The present invention relates to antisense oligonucleotides that modulate the expression of and / or function of Hepatocyte Growth Factor (HGF), in particular, by targeting natural antisense polynucleotides of Hepatocyte Growth Factor (HGF). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of HGF.

Description

FIELD OF THE INVENTION[0001]The present application claims the priority of U.S. provisional patent application No. 61 / 289,647 filed Dec. 23, 2009 which is incorporated herein by reference in its entirety.[0002]Embodiments of the invention comprise oligonucleotides modulating expression and / or function of HGF and associated molecules.BACKGROUND[0003]DNA-RNA and RNA-RNA hybridization are important to many aspects of nucleic acid function including DNA replication, transcription, and translation. Hybridization is also central to a variety of technologies that either detect a particular nucleic acid or alter its expression. Antisense nucleotides, for example, disrupt gene expression by hybridizing to target RNA, thereby interfering with RNA splicing, transcription, translation, and replication. Antisense DNA has the added feature that DNA-RNA hybrids serve as a substrate for digestion by ribonuclease H, an activity that is present in most cell types. Antisense molecules can be delivered...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K31/713A61P9/10A61P9/00A61P9/08A61P9/04A61P25/00A61P25/02A61P25/08A61P25/28A61P25/16A61P35/00A61P37/00A61P1/16A61P13/12A61P21/00A61P17/00A61P11/00A61P1/00A61P19/02A61P7/04A61P1/04A61P31/04A61P31/18A61P19/08A61P17/02A61P7/00A61P3/00A61P15/00A61P13/02A61P19/04A61P17/14A61P17/08C12N5/071G01N25/04C12N15/113
CPCC12N15/113C12N2310/14C12N2310/113C12N15/1136A61P1/00A61P1/04A61P1/16A61P3/00A61P7/00A61P7/02A61P7/04A61P9/00A61P9/04A61P9/08A61P9/10A61P11/00A61P13/02A61P13/12A61P15/00A61P17/00A61P17/02A61P17/04A61P17/08A61P17/14A61P19/02A61P19/04A61P19/08A61P21/00A61P25/00A61P25/02A61P25/08A61P25/14A61P25/16A61P25/28A61P29/00A61P31/04A61P31/18A61P35/00A61P37/00A61P37/02A61P37/06A61P37/08A61P43/00A61K31/7088A61K31/713A61K48/00C12Q1/6876C12Q2600/136C12N2310/31C12N2310/311C12N2310/3125C12N2310/313C12N2310/314C12N2310/315C12N2310/316C12N2310/321C12N2310/322C12N2310/3231
Inventor COLLARD, JOSEPHKHORKOVA SHERMAN, OLGA
Owner CURNA INC
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