50 results about "RNA splicing" patented technology

The invention relates to methods and reagents for influencing alternative RNA splicing in living cells. More particularly, the invention relates to novel means for influencing RNA splice choice in living cells using polynucleotide-based reagents that compete for binding sites in nucleotide binding proteins, and novel methods for using these reagents as therapeutics.

Disclosed are methods and compositions for detection and amplification of nucleic acids, wherein two DNA strands hybridized to an RNA strand are ligated. In one aspect, the disclosed methods include removal of an energy source, such as ATP, upon charging a ligase to form an enzyme-AMP intermediate, and then adding substrate, which results in one complete round of RNA-templated DNA ligation. In another aspect, the ligation reaction is accomplished by use of a mixture of at least two different ligase enzymes. The disclosed methods and compositions for RNA-templated DNA ligation may be particularly useful for detection of RNA sequence variants, for example RNA splice variants, and for quantitative expression analysis.

This invention relates to drug delivery and specifically to the preparation and use of functionalized carriers such as nanopolymers and nanovesicles for improved delivery of nucleic acid agents (NAA) to tissues and cells. These compounds have broad applicability for treating numerous diseases and disorders, including neurodegenerative and neuromuscular disorders. The concept encompasses preferably polymeric carriers for delivery of a class of oligonucleotides that modulate RNA splicing.

Disclosed herein are compounds, compositions and methods for modulating splicing of SMN2 mRNA in a cell, tissue or animal. Also provided are uses of disclosed compounds and compositions in the manufacture of a medicament for treatment of diseases and disorders, including spinal muscular atrophy.

The present invention provides novel analogs of FR901464, as well as an improved methodology for preparing FR901464 and its analogs. These compounds display an anti-cancer activity and are candidates for therapies against a number of disease states associated with dysfunctional RNA splicing.

The present invention relates to a method for screening and identifying compounds that modulate the activity of one or more components in the tRNA splicing pathway. In particular the invention relates to a method for screening and identifying compounds that modulate the activity tRNA splicing endonuclease and/or tRNA splicing ligase. The invention provides assays for the identification of compounds that inhibit animalia tRNA splicing endonuclease and/or animalia tRNA splicing ligase. The invention also provides assays for the identification of compounds that inhibit fungal tRNA splicing endonuclease and/or fungal tRNA splicing ligase. The methods of the present invention provide a simple, sensitive assay for high-throughput screening of libraries of compounds to identify pharmaceutical leads useful for treating and/or preventing cancer and/or fungal infections.

The invention discloses a lentiviral vector suitable for gene therapy of thalassemia and sickle anemia. The lentiviral vector comprises a beta globin expression cassette; the expression cassette comprises a micro-locus control area, a gene sequence of a beta globin, a promoter sequence of a flank of the upstream of the gene sequence of the beta globin, and a flanking sequence of the downstream ofthe gene sequence of the beta globin; the micro-locus control area is a micro-control element which is screened out from the locus control area of beta globin 16kb and does not contain an HS1 area. Compared with the prior art, the screened beta globin expression cassette has both efficiency and specificity, and does not cause reduction of lentivirus titer; a screened insulator sequence which comesfrom foamy virus and is only 36 bp does not contain a hidden RNA splicing signal, and has the functions of maintaining gene expression and preventing gene activation in a region where the insulator sequence is located; the lentiviral vector can be used for gene therapy of thalassemia and sickle anemia.

The present invention relates to riboswitches that have been engineered to regulate pre-mRNA splicing. In particular, the insertion of a high affinity theophylline binding aptamer into the 3′ splice site region, 5′ splice site region, or branchpoint sequence (BPS) of a pre-mRNA modulates RNA splicing in the presence of theophylline. Accordingly, the aspects of the present invention include, but are not limited to, theophylline-dependent riboswitches which modulate RNA splicing, methods of modulating RNA splicing using theophylline and its corresponding riboswitches, methods of improving/identifying theophylline-dependent riboswitches, methods of treating diseases associated with or caused by abnormal RNA splicing.

A method of regulating RNA splicing by inducing splice site base mutation or polypyrimidine region base substitution is disclosed, the method comprises expressing targeted cytidine deaminase in cellsto induce AG to AA mutation of 3' splicing site of intron of interested of gene of interested in the cells, or GT to AT mutation of 5' splicing site of the intron of interested of the gene of interested in the cells, or respective mutation of multiple C in a polypyrimidine region of the intron of interested of the gene of interested to T. The method can specifically block the exon recognition process, regulates the alternative splicing process of endogenous mRNA, induces exon skipping, activates alternative splicing sites, induces mutual exclusive exon conversion, induces intron retention andenhances exon retention.

The invention discloses a pcMINI vector as well as a construction method and application thereof. The pcMINI vector is formed by modifying a vector pcDNA3.1+, the pcMINI vector comprises a CMV enhancer, a PCMV promoter, a multiple cloning site (MCS), an exon-intron gene fragment and a BGH_PA_terminator terminator, and the multiple cloning site (MCS) is used for being inserted into a gene fragment with mutation at an exon-intron combination position. According to a pcMINI vector system, detection on different gene mutations can be achieved only by replacing inserted DNA fragments, the application range is wide, and the operation is easy and convenient; according to the constructed pcMINI vector, dNA fragments needing to be inserted are smaller, and vector construction is more efficient and faster; and meanwhile, an intron element of the pcMINI vector comprises an efficient splicing acceptor and a splicing donor site, so that effective RNA splicing of an inserted DNA fragment can be ensured. The invention provides a complete and feasible prokaryotic mRNA abnormal splicing verification method based on the pcMINI vector.

The present invention provides methods for analyzing exon expression profiles of a cell or type of cell. In the invention, the expression levels of a plurality of individual exons or multiexons for each of a plurality of genes in the genome of an organism are measured and analyzed to determine the biological state, such as the exon expression state or transcriptional state, of the cell or type of cell. The methods of the invention are useful for determination of alternative RNA splicing in a plurality of genes. The invention also provides nucleic acid probe arrays for determining in parallel the expression levels of a plurality of exons or multiexons for each of a plurality of genes in the genome of an organism. The invention further provides methods for determining the effects of perturbations, such as perturbations by drugs, on exon expression and alternative RNA splicing pathways.

The present invention provides novel analogs of FR901464, as well as an improved methodology for preparing FR901464 and its analogs. These compounds display an anti-cancer activity and are candidates for therapies against a number of disease states associated with dysfunctional RNA splicing.

The invention provides a multi-camera data splicing method based on a strict imaging model. The method comprises steps that an ideal multi-camera splicing focal plane is established according to design parameters of at least two cameras; according to the ideal splicing focal plane and ideal auxiliary parameters, a strict imaging model after multi-camera splicing is established; according to actual parameters, a strict imaging model of each camera is established; according to the strict imaging models of the cameras, a positive and negative computation model between image points and ground points is established; after multi-camera splicing, image points of the cameras corresponding to any image point on an image are calculated, re-sampling is further carried out, after multi-camera splicing, an image after splicing is outputted from the ideal image plane. Through the method, multi-camera splicing precision can be improved, problems of low multi-camera splicing precision and poor stability in the prior art are solved, through experiment verification, splicing precision is stable, splicing is not influenced by an image texture status factor, and the splicing precision is high.

The invention relates to an event-driven large screen display method suitable for multi-screen splicing. The method includes a method for changing the content of a functional area with a mouse dragging mode and a method for inputting parameters of the functional area with the mouse dragging mode. According to the method for changing the content of the functional area with the mouse dragging mode, a functional picture is dragged into a functional display area with the mode of clicking the functional picture on a tool bar through a mouse, and the content of the functional display area can be immediately changed into the function represented by the functional picture. According to the method for inputting the parameters of the functional area with the mouse dragging mode, a user drags an element in one functional area to another functional area capable of receiving the parameters, the functional area can recognize the parameter meaning represented by the dragged icon, and the analysis operation is carried out. According to the event-driven large screen display method, the large screen display purpose is finished through the mouse operation, a good user experience is provided for the user, and the problem that all large screens cannot be coordinated or be linked when different functions are displayed in the prior art is solved.

The present invention relates to a genetic variant of CMV, said genetic variant lacking intron 2 of the IE region of CMV (CMV IEΔi2) The present invention also relates to various uses of this genetic variant as well as RNA splice variants transcribed therefrom, and proteins expressed from the RNA splice variants, such as in the diagnosis of a CMV related cancer disease, and identification of individuals at risk of developing cancer or risk of transferring the CMV IEΔi2 virus with a human sample and prevention and treatment through targeting of unique CMV IE proteins for immunotherapy and vaccination.

The invention discloses a repeated positioning precision detection method of a white vehicle body main splicing clamp switching system. The method comprises following steps of a, selecting multiple to-be-tested points on a clamp substrate of the main splicing, and fixedly arranging multiple probes corresponding to the to-be-tested points; b, using a displacement sensor to measure a distance value between each to-be-tested point and the corresponding probe; c, repeating the step b and acquiring distance values between multiple groups of to-be-tested points and the corresponding probes; d, calculating the absolute position precision Xij of each to-be-tested point and the ideal position thereof; e, calculating repeated positioning precision R of the main splicing; and f, comparing the calculated repeated positioning precision R with the theoretical precision value, and judging whether the working state of the main splicing is normal or not. According to the invention, real-time detection can be conducted on the repeated positioning precision of the main splicing clamp switching system; obtained results are displayed on a computer in forms of graphs and data; and the system is capable of judging whether to give an alarm about stopping lines by judging whether the repeated positioning precision is in an error permissible range, thereby ensuring that the vehicle body meets a precision requirement.

The invention provides an SNV detection system affecting RNA splicing. The SNV detection system comprises the following steps of: step 1), according to locus information and genome sequence information of an SNP file, extracting upstream and downstream sequences at 100bp and a reverse complementary sequence of the locus before and after mutation; step 2), using the sequences extracted in step 1) to respectively predict splice loci based on three different methods, that is, a maximum entropy principle, a Markov model and an artificial neural network; and step 3), integrating according to a prediction score result of the three methods, and screening out the SNV having an effect on the RNA splicing. According to the SNV detection system affecting the RNA splicing provided by the invention, changes of the RNA splicing before and after mutation are predicted by integrating these three methods and taking SNV data as input, and the SNV affecting the RNA splicing is acquired, thereby effectively improving the prediction accuracy and providing reference for biological experiments and clinical researches.

Methods and products for the repair of genetically defective DNA in the region of a mutated exon, for the specific destruction of tumor cells, and for the identification of naturally trans-spliced RNA. The methods inter alia are based on the utilization of cellular RNA splicing components.

Compounds and methods for regulation of exonic splicing enhancers and exonic splicing silencers. Compounds include polynucleotides targeted to aberrant exonic splicing enhancers and exonic splicing silencers. Compounds and methods for the diagnosis of diseases and conditions associated with aberrant exonic splicing enhancers and exonic splicing silencers. Methods for identifying splicing-sensitive disease mutations, and functional RNA elements as targets for amelioration of aberrant pre-mRNA splicing.

The invention provides a method of on-site order-splicing based on physical supermarkets, which comprises the following steps of: displaying order splicing information; displaying commodity information for order splicing by a display device of a movable shopping cart, wherein, the order splicing commodity information comprises a quantity threshold value of the goods to be purchased; the commodityidentification device of the mobile shopping cart collects the commodity identification information of the purchased commodity for order splicing and obtains a pre- order splicing list, and the wireless communication module of the mobile shopping cart sends the order splicing list to the supermarket management background system. The supermarket management background judges whether the total pre-purchased quantity reaches the threshold value of the order splicing goods according to the order splicing list. If so, the pre-purchased list is sent to the mobile terminal, and the mobile terminal performs settlement and obtains the settlement list according to the pre-purchased list. The method of on-site order-splicing based on physical supermarkets enables different consumers in the supermarketto perform on-site order splicing on the purchase information of the same goods to be order, and consumers do not need to purchase a plurality of goods to be order at one time to purchase the goods at the promotion price.