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Storage-stable liquid detergent or cleaning agent containing protease and amylase

a technology of protease and amylase, which is applied in the direction of detergent compositions, detergent compounding agents, enzymology, etc., can solve the problems of insufficient storage stability, loss of considerable degree of enzymatic activity, washing or cleaning agent no longer exhibits the optimum cleaning performance,

Active Publication Date: 2014-10-02
HENKEL KGAA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent describes a liquid cleaning agent containing a protease and an amylase. The protease is designed to have a specific amino acid sequence and amino acid substitutions that provide optimal activity in the cleaning agent. The use of this specific protease and amylase combination results in a more effective cleaning process compared to using just the amylase alone.

Problems solved by technology

A disadvantage of prior art protease- and amylase-containing liquid washing and cleaning agents is that they have inadequate storage stability and they accordingly lose a considerable degree of enzymatic, in particular amylolytic and / or proteolytic, activity after only a short time.
The washing or cleaning agent then no longer exhibits optimum cleaning performance.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example

Determination of the Storage Stability of a Liquid Automatic Dishwashing Agent According to the Invention

[0215]The base formulation was an amylase-containing biphasic liquid automatic dishwashing agent which was of the following composition (all values stated in weight percent):

(a) Enzyme Phase:

[0216]

Builder18.0Sugar alcohol12.0Nonionic surfactant (C8-C105.0fatty alcohol ethoxylate with 22 EO)Alkali compound (base)3.5Boric acid3.0Phosphonate (HEDP)1.5Amylase1.2Ca salt1.2Zn salt0.2Thickener1.0Dye, perfume, preservative0.3Waterad 97

[0217]The amylase present was an α-amylase variant which, relative to the α-amylase AA560 according to SEQ ID no. 4, has the following sequence modifications in the numbering of α-amylase AA560: R118K, D183* (deletion), G184* (deletion), N195F, R320K, R458K (Novozymes).

(b) Alkaline Phase:

[0218]

Builder12.0Sodium carbonate10.0Sulfopolymer7.0Alkali compound (base)4.0Monoethanolamine3.5Phosphonate (HEDP)4.0Thickener1.0Dye, perfume, preservative0.3Waterad 100

[02...

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Abstract

The intention is to improve storage stability in a liquid washing or cleaning agent which contains a protease and amylase. This is achieved by using a protease which comprises an amino acid sequence which, over the entire length thereof, is at least 70% identical to the amino acid sequence stated in SEQ ID no. 1 and, in the numbering according to SEQ ID no. 1, has the amino acid substitution R99E or R99D in combination with at least two further amino acid substitutions which are selected from the group consisting of S3T, V4I and V199I.

Description

FIELD OF THE INVENTION[0001]The present invention generally relates to liquid washing and cleaning agents, and more particularly relates to liquid enzyme-containing washing and cleaning agents which contain defined proteases in combination with an amylase, and furthermore proposes methods in which such agents are used. The invention furthermore relates to uses of defined proteases in liquid washing or cleaning agents which contain an amylase.BACKGROUND OF THE INVENTION[0002]The use of proteases of the subtilisin type is preferred for washing and cleaning agents. The proteases used in the washing or cleaning agents known from the prior art either originally originate from microorganisms, for instance of the genera Bacillus, Streptomyces, Humicola, or Pseudomonas, and / or are produced by suitable microorganisms using per se known biotechnological methods, for instance by transgenic expression hosts from the genera Bacillus or by filamentous fungi.[0003]Further enzymes, in particular am...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C11D3/386
CPCC11D3/38618C12N9/54C11D3/386
Inventor MUSSMANN, NINAEITING, THOMASBASTIGKEIT, THORSTENBENDA, KONSTANTINHELLMUTH, HENDRIK
Owner HENKEL KGAA