Administration of tailored feedstock to increase antibiotic susceptibility

Abstract

A method for increasing susceptibility of microorganisms to antibiotics includes providing a microorganism; administering a single type of an antibiotic including a nitroimidazole compound to the microorganism; and administering any of an uronic, aldonic, ulosonic, and aldaric feedstock to the microorganism. The feedstock is adapted to promote cell metabolism and antibiotic activation, and inhibit antibiotic inactivation pathways in the microorganism causing increased sensitivity of the microorganism to the nitroimidazole.

Description

GOVERNMENT INTEREST[0001]The embodiments herein may be manufactured, used, and / or licensed by or for the United States Government without the payment of royalties thereon.BACKGROUNDTechnical Field[0002]The embodiments herein generally relate to a method for increasing susceptibility of microorganisms to antibiotics. The embodiments herein also relate to a method for enhancing susceptibility of tumor cells to nitroimidazole.Description of the Related Art[0003]Within this application there are several patents and publications that are referenced. The disclosures of all these patents and publications, in their entireties, are hereby expressly incorporated by reference into the present application.[0004]Nitroimidazoles are a class of chemical compounds with active imidazole ring and nitro group at 2′- or 5′-positions. Nitroimidazole antibiotics may be classified according to the location of the nitro group. Drugs of the 5-nitro variety include metronidazole, tinidazole, nimorazole, dime...

AI Technical Summary

Benefits of technology

The patent text describes a method for increasing the sensitivity of microorganisms and tumor cells to antibiotics. This is achieved by administering a nitroimidazole compound and a specific type of antibiotic, along with a feedstock that promotes cell metabolism and antibiotic activation, while inhibiting antibiotic inactivation pathways. The feedstock can be a sugar acid or a combination of sugar acids, and can be administered as a supplement for oral antibiotics or through injection. The method may also involve metabolizing gluconate from the glucuronic acid and controlling the production of NADH and NADPH in the cells. Overall, this method enhances the effectiveness of antibiotics and antitumor agents.

Problems solved by technology

The electron reduction results in free radical formation that leads to covalent modification of nucleic acids and essential proteins, thereby inactivating them, leading to cell death and causes oxidative damages to cellular components.

There are no current methods or additives to increase susceptibility of cells to antibiotics, such as nitroimidazoles, unless an additional antibiotic is administered.

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