Method for producing coniothyrium minitans conidium

Abstract

The invention provides a method for producing coniothyrium minitans conidium which takes agricultural wastes as raw materials. The method comprises the following steps: (1) pulverizing and drying the agricultural wastes; (2) preparing a matrix mixture; (3) preparing spore suspension; (4) inoculating; and (5) culturing. In the invention, the coniothyrium minitans conidium is prepared from the agricultural wastes; and after measurement, the final spore yield reaches 1.58*109-6.0*109pcs / g matrixes and has no significant difference with the spore yield by single wheat fermentation. The method overcomes the defect of high cost in the prior art, has very low cost and very good production effect, and can make use of the agricultural wastes.

Description

technical field

[0001] The invention relates to a method for producing conidia of shell mold. Background technique

[0002] Shell mold is a biocontrol fungus for controlling Sclerotinia sclerotiorum, which can be used as a high-efficiency biopesticide. The first prerequisite for the application of C. shield in production practice is to obtain a large number of active conidia. Studies have found that C. shieldiae does not produce conidia under liquid fermentation conditions, and produces little or no conidia under semi-solid conditions; using PDA plates for solid culture of C. shieldiae can produce a large number of conidia, but this production The method is inconvenient for industrial expansion and cultivation production. There is another method, which is to use wheat, oats, barley, etc. as substrates to cultivate C. shield, because these substrates contain a large amount of carbon and nitrogen sources, which can make C. shield produce a large number of conidia, and can be...

Examples

Embodiment 1

[0014] A method for producing conidia of shell mold, comprising the steps of:

[0015] (1) Substrate preparation: crush corn stalks so that the length of the waste particles after crushing does not exceed 8mm, and dry them in a dryer at 50°C to constant weight;

[0016] (2) Weighing the matrix and the fermentation broth according to the weight ratio of the matrix and the fermentation broth being 1:4, mixing them uniformly, placing them for 4 hours, sterilizing with damp heat for 1 hour, and cooling to obtain the matrix mixture;

[0017] (3) Preparation of spore suspension: get the shell mold after activation and culture, wash the conidia with water, and dilute the number of spores to be 1×10 6 Individual / ml spore suspension;

[0018] (4) Inoculation: draw 1ml of spore suspension and put it into the matrix mixture prepared in step (2), shake and mix, and place in a biochemical incubator for cultivation;

[0019] (5) Cultivation: Control the culture conditions to be 20°C and d...

Embodiment 2

[0023] The used agricultural waste is bran, the weight ratio of substrate and fermented liquid is 1: 5, the standing time is 3 hours, and the cultivation time is 20 days, all the other are the same as Example 1, and the amount of spores obtained is 5.8×10 9 per gram of matrix.

Embodiment 3

[0025] The agricultural waste used is mixed with chaff and rice straw in a ratio of 1:1, the weight ratio of substrate and fermentation liquid is 1:3, the standing time is 6 hours, and the cultivation time is 26 days. The rest are the same as in Example 1 to obtain has a spore count of 6.0×10 9 per gram of matrix.

technical field

[0001] The invention relates to a method for producing conidia of shell mold. Background technique

[0002] Shell mold is a biocontrol fungus for controlling Sclerotinia sclerotiorum, which can be used as a high-efficiency biopesticide. The first prerequisite for the application of C. shield in production practice is to obtain a large number of active conidia. Studies have found that C. shieldiae does not produce conidia under liquid fermentation conditions, and produces little or no conidia under semi-solid conditions; using PDA plates for solid culture of C. shieldiae can produce a large number of conidia, but this production The method is inconvenient for industrial expansion and cultivation production. There is another method, which is to use wheat, oats, barley, etc. as substrates to cultivate C. shield, because these substrates contain a large amount of carbon and nitrogen sources, which can make C. shield produce a large number of conidia, and can be...

Examples

Embodiment 1

[0014] A method for producing conidia of shell mold, comprising the steps of:

[0015] (1) Substrate preparation: crush corn stalks so that the length of the waste particles after crushing does not exceed 8mm, and dry them in a dryer at 50°C to constant weight;

[0016] (2) Weighing the matrix and the fermentation broth according to the weight ratio of the matrix and the fermentation broth being 1:4, mixing them uniformly, placing them for 4 hours, sterilizing with damp heat for 1 hour, and cooling to obtain the matrix mixture;

[0017] (3) Preparation of spore suspension: get the shell mold after activation and culture, wash the conidia with water, and dilute the number of spores to be 1×10 6 Individual / ml spore suspension;

[0018] (4) Inoculation: draw 1ml of spore suspension and put it into the matrix mixture prepared in step (2), shake and mix, and place in a biochemical incubator for cultivation;

[0019] (5) Cultivation: Control the culture conditions to be 20°C and d...

Embodiment 2

[0023] The used agricultural waste is bran, the weight ratio of substrate and fermented liquid is 1: 5, the standing time is 3 hours, and the cultivation time is 20 days, all the other are the same as Example 1, and the amount of spores obtained is 5.8×10 9 per gram of matrix.

Embodiment 3

[0025] The agricultural waste used is mixed with chaff and rice straw in a ratio of 1:1, the weight ratio of substrate and fermentation liquid is 1:3, the standing time is 6 hours, and the cultivation time is 26 days. The rest are the same as in Example 1 to obtain has a spore count of 6.0×10 9 per gram of matrix.