The invention relates to the technical field of blood sampling equipment, and discloses a blood sampling tube for vascular surgery, which comprises a mounting base, a collecting tube is fixedly mounted on the mounting base, the upper and lower parts of the collecting tube are opened, the bottom end of the collecting tube is connected with a negative pressure mechanism through a mounting rod, the negative pressure mechanism comprises a first sleeve, and the first sleeve is fixedly mounted on the mounting rod; a second sleeve is slidably mounted in the first sleeve, a first spring sleeves the first sleeve and the second sleeve, a piston is mounted at the top end of the second sleeve and slidably mounted in the collecting pipe in a sealed manner, the bottom end of the piston is connected with a driving mechanism through a connecting cable, a stirring mechanism is mounted in the middle of the piston in an embedded manner, and a sealing mechanism is installed at the top end of the collecting pipe through a fixing mechanism and is provided with a balance mechanism. The negative pressure state of the collecting pipe can be adjusted, meanwhile, the collecting amount is adjusted, automatic oscillation can be achieved, dismounting and cleaning are convenient, and energy-saving and environment-friendly performance is realized.