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The invention relates to a divisional application of a Chinese invention patent application with an application number of 202311209611. 2, an application date of September 19, 2023, and an invention name of a bio-organic fertilizer produced by using sugar industry byproducts and a preparation method thereof. The invention discloses an application of a bio-organic fertilizer produced by using by-products in the sugar industry in improvement of tomato yield and / or quality, and belongs to the technical field of bio-organic fertilizers. The invention provides an application of a bio-organic fertilizer produced by using by-products in the sugar industry in improvement of tomato yield and / or quality. The bio-organic fertilizer produced by utilizing the by-product of the sugar industry is prepared from the following components in parts by mass: 30 to 40 parts of water-soluble calcium humate, 20 to 35 parts of filter mud fermentation product, 15 to 25 parts of magnesium nitrate hexahydrate, 0.4 to 1 part of boric acid, 0.4 to 1 part of zinc sulfate, 1 to 2 parts of sodium molybdate, 1 to 2 parts of amino acid, 1 to 2 parts of dioctyl sodium sulfosuccinate, 0.5 to 1 part of D-glucuronic acid and 1 to 2 parts of sodium polyacrylate. The yield, quality and appearance of the tomatoes can be improved to different degrees by respectively spraying the bio-organic fertilizer in the early flowering stage, the fruit swelling stage and the color changing stage of the tomatoes.