Indoor hardening off method for poplar tissue cultivating seedling

A technology for tissue culture and hardening of seedlings, which is applied in cultivation, application, agriculture, etc., and can solve the problems of easy water loss and wilting of hardened seedlings, and the impact on survival rate

Inactive Publication Date: 2007-11-14
上海光兆植物速生技术有限公司
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Problems solved by technology

After the poplar tissue culture seedlings have been rooted and cultivated, they are directly transplanted into the greenhouse for hardening, which is easy to lose water and wilt, and the survival rate is affected

Method used

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Embodiment Construction

[0006] A kind of indoor seedling hardening method of poplar tissue culture seedling of the present invention is that the plant height of the poplar tissue culture seedling that has taken root is cultivated to more than 3 centimetres, and then cultivates 7 days indoors again, and the cultivation condition is: daily light time is 14 Hours, light intensity 3000-5000Lux, during the 7-day indoor seedling hardening process, gradually open the culture bottle cap, and keep the air humidity above 60%; on the first day, loosen the bottle cap; on the second day, open a 0.5 cm gap , and during the 14-hour light period, spray water mist on the tissue culture seedlings from the gap every 2 hours; on the third day, open a 1 cm gap, and spray from the gap every 2 hours; Spray once every 3 hours from the crevice; on the fifth day, open 1 / 3 of the gap of the bottle mouth, and spray once from the crevice every 3 hours; on the sixth day, spray once from the crevice every 4 hours; on the seventh da...

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Abstract

The present invention relates to tissue culture for fast propagation of plant, and is especially the indoor hardening off process of tissue cultured poplar seedling. The indoor hardening off process before transplantation of tissue cultured poplar seedling into green house includes culturing rooted tissue cultured poplar seedling to over 3 cm in abacterial condition, then indoor culture under the conditions of illumination intensity of 3000-5000 Lux for 7 days for over 14 hr each day and air humidity over 60 %, and gradually opening the bottle cap in the 7 days. The seedling after indoor hardening off is then transplanted into green house, and the said process can ensure a survival rate over 90 %.

Description

technical field [0001] The invention relates to a plant tissue culture rapid propagation technology, in particular to a method for indoor seedling hardening of poplar tissue culture seedlings. Background technique [0002] The plant tissue culture process includes a series of processes such as explant collection, medium preparation, filling, disinfection, storage, inoculation, and cultivation. After the poplar tissue-cultured seedlings have been rooted and cultured, they are directly transplanted into greenhouses for hardening, which is prone to water loss and wilting, and the survival rate is affected. In order to ensure the survival rate of poplar tissue-cultured seedlings for seedling hardening in greenhouses, the invention provides an indoor seedling hardening method. Contents of the invention [0003] The object of the present invention is to provide an indoor seedling hardening method for poplar tissue culture seedlings, so as to ensure the survival rate of transpla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G17/00
Inventor 宋学孟庞瑞华王凌健陈权董举文何燕红
Owner 上海光兆植物速生技术有限公司
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