Recombinant microorganism

Abstract

It is intended to provide a host microorganism capable of increasing productivity of a protein or a polypeptide; a recombinant microorganism obtained by introducing a gene encoding a protein or a polypeptide into the host microorganism; and a process for producing a protein or a polypeptide using the recombinant microorganism. The recombinant microorganism is prepared by using a microorganism, in which aprX gene of Bacillus subtilis or a gene corresponding to the gene has been deleted or inactivated, as a host and introducing a gene encoding a heterologous protein or polypeptide into the host. The recombinant microorganism is used in the process for producing a protein or a polypeptide.

Description

Technical field

[0001] The present invention relates to a microorganism that can be effectively used for protein or polypeptide production, and a method for producing the protein or polypeptide. Background technique

[0002] The industrial production of useful substances using microorganisms, represented by alcoholic beverages, soy sauce, soy sauce and other foods, covers amino acids, organic acids, nucleic acid-related substances, antibiotics, carbohydrates, lipids, proteins, etc., and its The application also extends to food, medicine, lotion, cosmetics and other daily necessities, or various chemical raw materials in a wide range of fields.

[0003] In this kind of industrial production of useful substances using microorganisms, improving their productivity is one of the important issues. The solution is to use mutations and other genetic methods to cultivate production bacteria. Especially recently, with the development of microbial genetics and biotechnology, genetic recombi...

Images