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Application of ophiopogoni D in preparing gene medicine for regulating vasular endothelial cell period

A technology of Ophiopogon japonicus saponins and drugs, applied in the application field of Ophiopogon japonicus saponin D in the preparation of gene drugs that regulate the cycle of vascular endothelial cells, and can solve the problems of low sensitivity, interference peaks, and inability to use

Inactive Publication Date: 2010-10-13
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0009] Because saponin is a kind of compound with large polarity and complex structure, it has no ultraviolet absorption and no specific chromogenic agent, and the determination of Ophiopogon japonicus saponin is often interfered by sugar. If the terminal absorption method of ultraviolet detection is used, the sensitivity Low, with interference peaks, unusable

Method used

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  • Application of ophiopogoni D in preparing gene medicine for regulating vasular endothelial cell period
  • Application of ophiopogoni D in preparing gene medicine for regulating vasular endothelial cell period
  • Application of ophiopogoni D in preparing gene medicine for regulating vasular endothelial cell period

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Embodiment

[0016] Example: Ophioside D regulates vascular endothelial cell cycle genes

[0017] The effective component of Radix Ophiopogon japonicus saponin D extracted from the Chinese herbal medicine Ophiopogon japonicus has anti-H effect on human umbilical vein endothelial cells. 2 o 2 effect on apoptosis. The invention utilizes the gene chip technology to discover the vascular endothelial cell gene expression profile regulated by Ophiopogon saponin D for cell cycle-related genes.

[0018] 1 Materials and methods

[0019] 1.1 Main reagents and instruments: RPMI-1640 medium (GIBCO, USA) (containing 20% ​​calf serum per liter, 0.33 mg of L-glutamine, 100 u of penicillin, 100 u of streptomycin, pH-7.4); the digestive agent is 0.25% trypsin (MERCK, USA); Radix Ophiopogon japonicus. (L.f.) Ker-Gawl. produced in Mianyang, Sichuan, purchased from Nanjing Medicinal Materials Company, identified as Ophiopogon japonicus. (L.f.) Ker-Gawl.

[0020] RNA extraction reagents: Reagents (Invitr...

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Abstract

The present invention is target cell period gene chip for the research of 120 genes related to cell period regulation. It is found by means of gene chip technology research that H2O2 damages human umbilical vein vascular endothelial cell via up regulating the expression of its 48 cell period genes while ophiopogonin D can down regulate the expression of 39 genes of the said 48 cell period genes and other 15 genes, and H2O2 can down regulate the expression of its 1 cell period gene while ophiopogonin D can down regulate the expression of this gene. Therefore, ophiopogonin D can antagonize the damage of H2O2 on vascular endothelial cell to protect the morphologic and functional integrity of vascular endothelial cell and to prevent and treat cardiac and cerebral vascular diseases.

Description

1. Technical field [0001] The invention relates to the new application of Ophiopogonis saponin D in pharmacy, in particular to the application of Ophiopogonis saponin D in the preparation of gene medicines regulating the cycle of vascular endothelial cells, which belongs to the technical field of traditional Chinese medicine. 2. Background technology [0002] 1. Ophiopogon japonicus completes the effect of nourishing yin and promoting blood circulation by protecting vascular endothelial cells [0003] Ophiopogon japonicus is a commonly used product for nourishing yin liquid clinically, and it is one of the representative drugs for nourishing yin and promoting body fluid. Ophiogon japonicus, the dried tuber root of Ophiogon japonicus (Thumb.) Ker-Gawl. It mainly contains a variety of glycosides: Ophioside B, D, 23,24 dihydroxyroscoside, Ophioside-3-O-a-L-rhamnopyranosyl (1-2)-β-D-pyridine Glucopyranoside, methyl philodendron flavanone A, B, ophiopogon flavanone A, B, 6-form...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61P9/00C12Q1/68C07H1/08
Inventor 张旭陆兔林蔡宝昌王明艳毛春芹张小燕张志洁杨进
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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