Preparation method of banana polysaccharide

Abstract

The invention discloses a preparation method of banana polysaccharide, and the preparation method comprises the following processes in sequence: the banana is washed to be clean, cut to pieces and soaked and processed with acid solution, and then blanched with hot water for a few minutes, cooled, and beaten for stand-by, and then water is added to be extracted. The hydrolysis is performed with double enzyme, and then the enzyme inactivation is performed; the liquid is filtered, and the clear liquid is alcoholized and settled statically over night. The liquid is centrifuged and filtered, the deposit is taken and then washed for two times respectively with 95 percent of ethyl alcohol and acetone, and then dried with vacuum, and the banana polysaccharide crude product is obtained. The banana polysaccharide crude product is taken to be dissolved, centrifuged and filtered with water, and then adsorbed, separated and purified through a DEAE chromatography column on the filtrate, the object is collected and then ultrafilted with ultra filtration membrane, and trapped fluid is collected, alcoholized and settled statically over night. The fluid is centrifuged and filtered, the deposit is washed for two times respectively with 95 percent of ethyl alcohol and acetone, and then dried with vacuum, and the high-purity banana polysaccharide is obtained. The extraction method is simple and safe, no pollution is produced, the yield is high, the production cost is low, the purity of the obtained banana polysaccharide is high, and the preparation method is suitable for the large-scale industrialization production.

Description

technical field

[0001] The invention relates to the production technology of food, medicine and chemical industry, in particular to the preparation method of banana polysaccharide. Background technique

[0002] In the field of life science research, glycobiology has always been the research frontier and hotspot in this field. Polysaccharides have complex biological activities and functions in many aspects. The sugar chains of polysaccharides can control cell division and differentiation, and regulate cell growth and aging.

[0003] The sugar content in ripe bananas is nearly 20%. Banana polysaccharides have a certain inhibitory effect on the decrease of leukocytes in mice caused by cyclophosphamide, that is, they have certain immune activity, which provides a scientific basis for the development and application of banana polysaccharides.

[0004] However, bananas are mostly eaten as a fruit now, and their medicinal value is often ignored. Banana polysaccharides have immune...

Examples

Embodiment

[0031] Take 200 ripe bananas, wash them, weigh them to 6.0kg, cut them into sections, soak them in a hydrochloric acid solution with a pH of 3.0-4.5, and then scald them with hot water at 100°C for 7 minutes, cool them down, and beat them for later use. Add 20.0 l of water and heat to 100°C for extraction for 1.5 hours, cool down to room temperature, and filter with filter cloth. Add 20.0 l of water to the banana pomace and heat to 100°C for extraction for 1.5 hours, cool down to room temperature, and filter with filter cloth. Collect the extract twice, centrifuge, filter, wait for the filtrate to cool down to 45 ℃ ~ 50 ℃, adjust the pH to 3.0 ~ 4.0 with 30% (w%) citric acid, add 100,000 international units (specific activity ) ratio of papain, add 500,000 international units (specific activity) of papain, stir well, and maintain pH 3.0-4.0, 45°C-50°C for 8 hours for hydrolysis. Use 18% (w%) NaOH to adjust the pH of the pepsin hydrolysis slurry to 9.0-9.5, add 200,000 interna...

technical field

[0001] The invention relates to the production technology of food, medicine and chemical industry, in particular to the preparation method of banana polysaccharide. Background technique

[0002] In the field of life science research, glycobiology has always been the research frontier and hotspot in this field. Polysaccharides have complex biological activities and functions in many aspects. The sugar chains of polysaccharides can control cell division and differentiation, and regulate cell growth and aging.

[0003] The sugar content in ripe bananas is nearly 20%. Banana polysaccharides have a certain inhibitory effect on the decrease of leukocytes in mice caused by cyclophosphamide, that is, they have certain immune activity, which provides a scientific basis for the development and application of banana polysaccharides.

[0004] However, bananas are mostly eaten as a fruit now, and their medicinal value is often ignored. Banana polysaccharides have immune...

Examples

Embodiment

[0031] Take 200 ripe bananas, wash them, weigh them to 6.0kg, cut them into sections, soak them in a hydrochloric acid solution with a pH of 3.0-4.5, and then scald them with hot water at 100°C for 7 minutes, cool them down, and beat them for later use. Add 20.0 l of water and heat to 100°C for extraction for 1.5 hours, cool down to room temperature, and filter with filter cloth. Add 20.0 l of water to the banana pomace and heat to 100°C for extraction for 1.5 hours, cool down to room temperature, and filter with filter cloth. Collect the extract twice, centrifuge, filter, wait for the filtrate to cool down to 45 ℃ ~ 50 ℃, adjust the pH to 3.0 ~ 4.0 with 30% (w%) citric acid, add 100,000 international units (specific activity ) ratio of papain, add 500,000 international units (specific activity) of papain, stir well, and maintain pH 3.0-4.0, 45°C-50°C for 8 hours for hydrolysis. Use 18% (w%) NaOH to adjust the pH of the pepsin hydrolysis slurry to 9.0-9.5, add 200,000 interna...