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Biological formulation product for treating habitual abortion

A habitual abortion and biological technology, applied in the field of medicine, achieves the effects of stable quality control, strong controllability, and simple clinical application

Inactive Publication Date: 2011-05-11
中国人民解放军陆军军医大学士官学校
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there has been no literature report about its use as an immune tolerance inducer for the treatment of habitual abortion, let alone a preparation for practical treatment.

Method used

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  • Biological formulation product for treating habitual abortion

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Experimental program
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Embodiment Construction

[0015] The invention is divided into two steps, one is the in vitro stimulation and cultivation of healthy adult male peripheral blood T cells, and the other is preparation of T cell exosomes by centrifugal ultrafiltration and sucrose density gradient centrifugation.

[0016] First, take 50ml of peripheral blood from a healthy adult male, defibrillate it with glass balls, dilute it 1.5 times, and separate and collect mononuclear cells with lymphocyte separation medium; ) to resuspend the cells in complete culture medium, count and adjust the cell concentration to 2×10 6 / ml, add phytohemagglutinin (PHA) to make the final concentration 100μg / ml; observe the transformation of T cells under the microscope after culturing at 37°C for 72 hours; replace with fresh culture medium containing PHA to stimulate and culture repeatedly. The collected culture supernatant can be stored at 4°C for future use.

[0017] Then prepare biological preparations, take in vitro stimulated and culture...

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Abstract

The invention relates to a biological agent for treating habitual abortion in the medicine technological field, which comprises the following steps: extracting peripheral blood of healthy adult men; diluting the blood to 1. 5 times through glass fibers from the ball and Hanks liquid to separate lymphocyte to collect single core cells; washing by Hanks liquid to culture and flow and count with absolute culture liquid of RPMI-1640; adjusting the cell concentration to 2*10<6> / ml to add phasecolosaxin to culture at 37 DEG C for 72hours; collecting supernatant to produce T cells through centrifuging of centrifugal ultrafiltration and cane sugar density gradient centrifugation; filtering by sterile filter membrane to be split charging to produce completed product at 80 DEG C below zero. The invention evokes immune tolerance of body effectively and overcomes the more faults of evoking immune tolerance through cell adoptive immunotherapy to make easier clinical application, which can be produced in a large industrialization scale to get more stabilizing mass control and higher controllability for treating the habitual abortion with good curative effect.

Description

technical field [0001] The invention relates to an immunological preparation for treating unexplained habitual abortion by using non-cellular components instead of traditional cell components, which belongs to the technical field of medicine. Background technique [0002] Habitual abortion refers to three or more consecutive abortions, the incidence rate is about 1% of the total number of pregnancies, accounting for 15% of the number of spontaneous abortions, and it is an obstetric disease that seriously endangers the physical and mental health of pregnant women. There have been cases of 7 consecutive miscarriages in clinical cases, and the patients are very painful. The etiology of this disease is very complex, including genetic factors, anatomical abnormalities, endocrine abnormalities, infection, etc. However, after excluding the above-mentioned clinical causes, there are still 59.42% of patients with habitual abortion, which is called URSA (Unexplanated habitual abortion...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61P15/06A61K35/14C12N5/08A61K35/17
Inventor 崔瀓杨彦忠
Owner 中国人民解放军陆军军医大学士官学校
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