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Reagent kit for detecting pulmonary cancer susceptibility with XRCC1 gene and CYP1A1 gene

A CYP1A1, kit technology, applied in biochemical equipment and methods, microbial determination/inspection, material excitation analysis, etc., can solve problems such as differences in enzyme activity

Inactive Publication Date: 2008-03-12
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme kinetic studies revealed differences in enzyme activity expressed by the 3 genotypes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Use of detection kits

[0028] Step 1: Extraction of DNA template

[0029] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0030] Step 2: Real-time quantitative PCR reaction

[0031] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:

[0032] Sense primer 1: 5'-TAACTGGCATCTTCACTTCT-3' (SEQ ID NO: 1) with a Tm value of 56°C

[0033] Antisense primer 1: 5'-TCCAGATTCCTGGCATTGC-3' (SEQ ID NO: 2) with a Tm value of 58°C

[0034] Sense primer 2: 5'-GTGTTAAGTGAGAAGGTGAT-3' (SEQ ID NO: 3) with a Tm value of 56°C

[0035] Antisense primer 2: 5'-AGGATAGCCAGGAAGAGAAA-3' (SEQ ID NO: 4) with a Tm value of 58°C

[0036] Fluorescent probe 1: 5'-CGGCTGCCCTCCCAGAGG-3' (SEQ ID NO: 5) with a Tm value of 64°C

[0037] Fluorescent probe 2: 5'-TATCGGTGAGACCGTTGCCC-3' (SEQ ID NO: 6) with a Tm value of 64°C

[0038] Sense primer 1, anti...

Embodiment 2

[0043] Example 2. Services that guide people to proactively prevent lung cancer

[0044] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0045] Step 1: DNA Extraction

[0046] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0047] Step 2: Genotyping Assays

[0048] Using the kit provided by the invention, simultaneously perform fluorescent quantitative PCR detection on the SNP site rs25487 on the XRCC1 gene and the SNP site rs1048943 on the CYP1A1 gene of the DNA of the subject to be tested, and determine the genotypes of the two SNPs sites.

[0049] Step 3: Instruct people to be proactive in preventing lung cancer

[0050] Through the analysis of...

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PUM

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Abstract

The present invention discloses a reagent kit to detect lung cancer susceptibility, which comprises a fluorescence probe to synchronously detect specificity tracers and specificity of No. rs25478 SNP locus on gene XRCC1 and No. rs1048943 SNP locus on gene CYP1A1, and a normal assembly to detect fluorescence quantitative PCR and so on. The reagent kit of the present invention synchronously detects and analyzes individual SNPs locus gene carrying type on gene XRCC1 and CYP1A1 to predict susceptibility of the individual to lung cancer.

Description

technical field [0001] The invention relates to a kit for detecting disease susceptibility, in particular to a kit for detecting susceptibility to lung cancer, through simultaneous detection of X-ray repair cross-complementing gene 1 (XRCC1) Single nucleotide polymorphism (SNP) site and cytochrome P4501A1 enzyme gene (cytochrome P450, family 1, subfamily A, polypeptide 1, CYP1A1) single nucleotide polymorphism site to predict the susceptibility of individuals to lung cancer emotional. Background technique [0002] Primary bronchial lung cancer, referred to as lung cancer, is one of the most common malignant tumors in the world and a serious threat to human health and life. Tumor cells originate from the bronchial mucosa or glands, and often have regional lymph node and hematogenous metastasis. In the early stage, respiratory symptoms such as irritating cough and blood in the sputum are often present. The speed of disease progression is related to the biological characterist...

Claims

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Application Information

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IPC IPC(8): G01N21/64C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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