Reagent kit for detecting lung cancer susceptibility through SNPs of PARP1 genes

A kit and susceptibility technology, applied in the determination/inspection of microorganisms, material analysis through optical means, measurement devices, etc., can solve the problems of inability to correctly identify DNA damage sites, carcinogenic damage accumulation, and base excision repair. proceeding and other issues

Inactive Publication Date: 2008-04-02
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Loss of function of ERCC2 results in the inability of the essential transcription factor complex to correctly recognize sites of DNA damage, thereby preventing base excision repair from proceeding correctly, leading to the accumulation of oncogenic lesions on DNA

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Use of detection kits

[0026] Step 1: Extraction of DNA template

[0027] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0028] Step 2: Real-time quantitative PCR reaction

[0029] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:

[0030] Sense primer 1: 5'-TTTGCCATTCACTGTGTTGG-3' (SEQ ID NO: 1) with a Tm value of 58°C

[0031] Antisense primer 1: 5'-ATCCTTGCTGCTATCATCA-3' (SEQ ID NO: 2) with a Tm value of 54°C

[0032] Sense primer 2: 5'-GTGTTAAGTGAGAAGGTGAT-3' (SEQ ID NO: 3) with a Tm value of 56°C

[0033] Antisense primer 2: 5'-AGGATAGCCAGGAAGAGAAA-3' (SEQ ID NO: 4) with a Tm value of 58°C

[0034] Sense primer 3: 5'-TAACTGGCATCTTCACTTCT-3' (SEQ ID NO: 5) with a Tm value of 56°C

[0035] Antisense primer 3: 5'-TCCAGATTCCTGGCATTGC-3' (SEQ ID NO: 6) with a Tm value of 58°C

[0036] Sense primer 4: 5'-ACTCAG...

Embodiment 2

[0047] Example 2. Services that guide people to proactively prevent lung cancer

[0048] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0049] Step 1: DNA Extraction

[0050] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0051] Step 2: Genotyping Assays

[0052] Using the kit provided by the invention, the SNP site No. rs1136410 on the PARP1 gene, the SNP site No. rs1048943 on the CYP1A1 gene, the SNP site No. rs25487 on the XRCC1 gene and the SNP site No. rs13181 on the ERCC2 gene Fluorescent quantitative PCR detection was performed at the same time to determine the genotypes of the four SNPs loci.

[0053] Step 3: Instruct people to be proact...

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Abstract

The invention discloses a reagent kit for detecting lung cancer susceptibility, comprising a common component for fluorescence quantitative PCR detection, and a specific primer pair and a specific fluorescent probe which detect the NO. rs1136410 SNP site of PARP1 gene, the NO. rs1048943 SNP site of CYP1A1 gene, the NO. rs25487 SNP site of XRCC1 gene and the NO. rs13181 SNP site of ERCC2 gene at the same time. The reagent kit can predict the lung cancer susceptibility of individual through detecting the SNPs site genotype of PARP1 gene, CYP1A1 gene, XRCC1 gene and ERCC2 gene of individual at the same time.

Description

technical field [0001] The present invention relates to a kit for detecting disease susceptibility, especially a kit for detecting susceptibility to lung cancer. , PARP1, also abbreviated as ADPRT), cytochrome P4501A1 enzyme gene (cytochrome P450, family 1, subfamily A, polypeptide 1, CYP1A1), X-ray repair cross--complementing gene 1 (XRCC1 ) and excision repair complex 2 (Excision Repair Cross-complementing Rodent Repair Deficiency, Complementation Group 2, ERCC2) single nucleotide polymorphism sites (SNP) to predict the susceptibility of individuals to lung cancer. Background technique [0002] Primary bronchial lung cancer, referred to as lung cancer, is one of the most common malignant tumors in the world and a serious threat to human health and life. Tumor cells originate from the bronchial mucosa or glands, and often have regional lymph node and hematogenous metastasis. In the early stage, respiratory symptoms such as irritating cough and blood in the sputum are often...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/17C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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