Kit for detecting lung cancer susceptibility by PARP1 gene, ERCC2 gene and XRCC1 gene
A kit and susceptibility technology, applied in the field of kits for detecting lung cancer susceptibility through PARP1 gene, ERCC2 gene and XRCC1 gene, can solve the problem of accumulation of carcinogenic damage, inability to correctly identify DNA damage sites, and base excision repair inaccuracy proceeding and other issues
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Embodiment 1
[0025] Example 1. Use of detection kits
[0026] Step 1: Extraction of DNA template
[0027] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.
[0028] Step 2: Real-time quantitative PCR reaction
[0029] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:
[0030] Sense primer 1: 5'-TTTGCCATTCACTGTGTTGG-3' (SEQ ID NO: 1) with a Tm value of 58°C
[0031] Antisense primer 1: 5'-ATCCTTGCTGCTATCATCA-3' (SEQ ID NO: 2) with a Tm value of 54°C
[0032] Sense primer 2: 5'-ACTCAGGAGTCACCAGGAA-3' (SEQ ID NO: 2) with a Tm value of 58°C
[0033] Antisense primer 2: 5'-TCTGTTCTCTGCAGGAGGA-3' (SEQ ID NO: 3) Tm value is 58°C
[0034] Sense primer 3: 5'-TAACTGGCATCTTCACTTCT-3' (SEQ ID NO: 5) with a Tm value of 56°C
[0035] Antisense primer 3: 5'-TCCAGATTCCTGGCATTGC-3' (SEQ ID NO: 6) with a Tm value of 58°C
[0036] Fluorescent probe 1: 5'-CAGGCCAAGG...
Embodiment 2
[0044] Example 2. Services that guide people to proactively prevent lung cancer
[0045] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.
[0046] Step 1: DNA Extraction
[0047] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.
[0048] Step 2: Genotyping Assays
[0049] Using the kit provided by the invention, the rs1136410 SNP site on the PARP1 gene of the tested person, the rs13181 SNP site on the ERCC2 gene and the rs25487 SNP site on the XRCC1 gene are simultaneously detected by fluorescence quantitative PCR to determine the Genotypes of the three SNPs loci.
[0050] Step 3: Instruct people to be proactive in preventing lung cancer
[0051] Throug...
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