Kit for detecting lung cancer susceptibility by PARP1 gene, ERCC2 gene and XRCC1 gene

A kit and susceptibility technology, applied in the field of kits for detecting lung cancer susceptibility through PARP1 gene, ERCC2 gene and XRCC1 gene, can solve the problem of accumulation of carcinogenic damage, inability to correctly identify DNA damage sites, and base excision repair inaccuracy proceeding and other issues

Inactive Publication Date: 2008-03-19
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Loss of function of ERCC2 results in the inability of the essential transcription factor complex to correctly recognize sites of DNA damage, thereby preventing base excision repair from proceeding correctly, leading to the accumulation of oncogenic lesions on DNA

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Use of detection kits

[0026] Step 1: Extraction of DNA template

[0027] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0028] Step 2: Real-time quantitative PCR reaction

[0029] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:

[0030] Sense primer 1: 5'-TTTGCCATTCACTGTGTTGG-3' (SEQ ID NO: 1) with a Tm value of 58°C

[0031] Antisense primer 1: 5'-ATCCTTGCTGCTATCATCA-3' (SEQ ID NO: 2) with a Tm value of 54°C

[0032] Sense primer 2: 5'-ACTCAGGAGTCACCAGGAA-3' (SEQ ID NO: 2) with a Tm value of 58°C

[0033] Antisense primer 2: 5'-TCTGTTCTCTGCAGGAGGA-3' (SEQ ID NO: 3) Tm value is 58°C

[0034] Sense primer 3: 5'-TAACTGGCATCTTCACTTCT-3' (SEQ ID NO: 5) with a Tm value of 56°C

[0035] Antisense primer 3: 5'-TCCAGATTCCTGGCATTGC-3' (SEQ ID NO: 6) with a Tm value of 58°C

[0036] Fluorescent probe 1: 5'-CAGGCCAAGG...

Embodiment 2

[0044] Example 2. Services that guide people to proactively prevent lung cancer

[0045] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0046] Step 1: DNA Extraction

[0047] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0048] Step 2: Genotyping Assays

[0049] Using the kit provided by the invention, the rs1136410 SNP site on the PARP1 gene of the tested person, the rs13181 SNP site on the ERCC2 gene and the rs25487 SNP site on the XRCC1 gene are simultaneously detected by fluorescence quantitative PCR to determine the Genotypes of the three SNPs loci.

[0050] Step 3: Instruct people to be proactive in preventing lung cancer

[0051] Throug...

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Abstract

The present invention discloses a reagent kit for detecting the susceptibility of the lung cancer. The reagent kit comprises a specific primer pair which detects the SNP locus of No. rs1136410 on a PARP1 gene, the SNP locus of No. rs13181 on an ERCC2 gene and the SNP locus of No. rs25487 on a XRCC1 gene synchronously, a specific fluorescent probe, and a conventional component which is used for the fluorescent quantitative PCR detection, etc. The reagent kit of the present invention predicts the susceptibility of the individual to the lung cancer through detecting the gene carrying type of the SNPs loci on the PARP1 gene, the ERCC2 gene and the XRCC1 gene of the individual synchronously.

Description

technical field [0001] The present invention relates to a kit for detecting disease susceptibility, especially a kit for detecting susceptibility to lung cancer, by simultaneously detecting poly(ADP-ribose) polymerase family, member1, PARP1, also abbreviated as ADPRT), excision repair complex 2 (Excision Repair Cross-complementing Rodent Repair Deficiency, Complementation Group2, ERCC2) and X-ray repair cross-complementing gene 1 (XRCC1) single nucleotide polymorphisms (SNPs) to predict an individual's susceptibility to lung cancer. Background technique [0002] Primary bronchial lung cancer, referred to as lung cancer, is one of the most common malignant tumors in the world and a serious threat to human health and life. Tumor cells originate from the bronchial mucosa or glands, and often have regional lymph node and hematogenous metastasis. In the early stage, respiratory symptoms such as irritating cough and blood in the sputum are often present. The speed of disease prog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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