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Kit for detecting lung cancer susceptibility by two different SNPs of ERCC2 gene

A kit and susceptibility technology, which is applied in the field of kits for detecting susceptibility to lung cancer, can solve problems such as decreased repair ability, decreased transcription factor, and weakened performance of transcription factor complexes

Inactive Publication Date: 2008-03-19
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A number of current studies have shown that the polymorphism of this site will weaken the performance of the transcription factor complex that ERCC2 participates in, thereby reducing the ability to repair DNA damage, and the ability of transcription factors will also decrease at the same time, resulting in a series of Occurrence of diseases such as lung cancer, prostate cancer, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Use of detection kits

[0026] Step 1: Extraction of DNA template

[0027] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0028] Step 2: Real-time quantitative PCR reaction

[0029] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:

[0030] Sense primer 1: 5'-ATCAAAGAGACAGACGAGCA-3' (SEQ ID NO: 1) with a Tm value of 58°C

[0031] Antisense primer 1: 5'-TCAGGAAGCCCAGGAAAT-3' (SEQ ID NO: 2) with a Tm value of 54°C

[0032] Sense primer 2: 5'-ACTCAGGAGTCACCAGGAA-3' (SEQ ID NO: 3) with a Tm value of 58°C

[0033] Antisense primer 2: 5'-TCTGTTCTTCTGCAGGAGGA-3' (SEQ ID NO: 4) Tm value is 58°C

[0034] Fluorescent probe 1: 5'-TGCCCAACGAAGTGCTGCAG-3' (SEQ ID NO: 5) with a Tm value of 64°C

[0035] Fluorescent probe 2: 5'-AATCTGCTCTTATCCTCTGCAGC-3' (SEQ ID NO: 6) with a Tm value of 66°C

[0036] Sense primer 1, antisense ...

Embodiment 2

[0041] Example 2. Services that guide people to proactively prevent lung cancer

[0042] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0043] Step 1: DNA Extraction

[0044] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0045] Step 2: Genotyping Assays

[0046] Using the kit provided by the invention, simultaneously perform fluorescent quantitative PCR detection on the rs1799793 SNP site and the rs13181 SNP site on the ERCC2 gene of the detected person's DNA, and determine the genotypes of the two SNPs sites.

[0047] Step 3: Instruct people to be proactive in preventing lung cancer

[0048] Through the analysis of the SNPs genotype of the tes...

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PUM

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Abstract

The present invention discloses a reagent kit for detecting the susceptibility of the lung cancer. The reagent kit comprises a specific primer pair which detects the SNP locus of No. rs1799793 and the SNP locus of No. rs13181 on an ERCC2 gene synchronously, a specific fluorescent probe, and a conventional component which is used for the fluorescent quantitative PCR detection, etc. The reagent kit of the present invention predicts the susceptibility of the individual to the lung cancer by detecting the gene carrying type of the two different SNPs loci on the ERCC2 gene of the individual synchronously.

Description

technical field [0001] The present invention relates to a kit for detecting disease susceptibility, especially a kit for detecting susceptibility to lung cancer. Two different single nucleotide polymorphisms (SNPs) to predict individual susceptibility to lung cancer. Background technique [0002] Primary bronchial lung cancer, referred to as lung cancer, is one of the most common malignant tumors in the world and a serious threat to human health and life. Tumor cells originate from the bronchial mucosa or glands, and often have regional lymph node and hematogenous metastasis. In the early stage, respiratory symptoms such as irritating cough and blood in the sputum are often present. The speed of disease progression is related to the biological characteristics of the cells. [0003] For half a century, the morbidity and mortality of lung cancer in all countries in the world have been increasing significantly. The World Health Organization (WHO) reported in 2000: In 1997, a ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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