Kit for detecting lung cancer susceptibility by one SNP on PARP1 gene and two SNPs on ERCC2 gene
A kit and susceptibility technology, applied in the field of kits for detecting lung cancer susceptibility through one SNP on the PARP1 gene and two SNPs on the ERCC2 gene, can solve the weakening of transcription factor complex performance, transcription factor decline, repair decline in ability
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Embodiment 1
[0027] Example 1. Use of detection kits
[0028] Step 1: Extraction of DNA template
[0029] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.
[0030] Step 2: Real-time quantitative PCR reaction
[0031] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:
[0032] Sense primer 1: 5'-TTTGCCATTCACTGTGTTGG-3' (SEQ ID NO: 1) with a Tm value of 58°C
[0033] Antisense primer 1: 5'-ATCCTTGCTGCTATCATCA-3' (SEQ ID NO: 2) with a Tm value of 54°C
[0034] Sense primer 2: 5'-ACTCAGGAGTCACCAGGAA-3' (SEQ ID NO: 3) with a Tm value of 58°C
[0035] Antisense primer 2: 5'-TCTGTTCTTCTGCAGGAGGA-3' (SEQ ID NO: 4) Tm value is 58°C
[0036] Sense primer 3: 5'-ATCAAAGAGACAGACGAGCA-3' (SEQ ID NO: 5) with a Tm value of 58C
[0037] Antisense primer 3: 5'-TCAGGAAGCCCAGGAAAT-3' (SEQ ID NO: 6) with a Tm value of 54°C
[0038] Fluorescent probe 1: 5'-CAGGCCAAGGC...
Embodiment 2
[0046] Example 2. Services that guide people to proactively prevent lung cancer
[0047] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.
[0048] Step 1: DNA Extraction
[0049] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.
[0050] Step 2: Genotyping Assays
[0051] Using the kit provided by the invention, the rs1136410 SNP site on the PARP1 gene, the rs13181 SNP site on the ERCC2 gene and the rs1799793 SNP site on the ERCC2 gene are simultaneously detected by fluorescence quantitative PCR to determine the Genotypes of the three SNPs loci.
[0052] Step 3: Instruct people to be proactive in preventing lung cancer
[0053] Through the analysis of t...
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