Chemically synthesized HSV1 virus gB glucoprotein extracellular region gene fragment, representation and application of the same

A technology of gene fragments and chemical synthesis, applied in the fields of vaccines and diagnostic reagents, and genetic engineering technology, can solve the problems of high risk and high production cost

Inactive Publication Date: 2010-10-27
李越希
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The research of inactivated vaccines has made great progress, but the production cost is high and the risk is great

Method used

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  • Chemically synthesized HSV1 virus gB glucoprotein extracellular region gene fragment, representation and application of the same
  • Chemically synthesized HSV1 virus gB glucoprotein extracellular region gene fragment, representation and application of the same
  • Chemically synthesized HSV1 virus gB glucoprotein extracellular region gene fragment, representation and application of the same

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Experimental program
Comparison scheme
Effect test

Embodiment approach

[0216] 1. Screening of gB glycoprotein epitopes of HSV1 virus and synthesis of gene fragments:

[0217] Utilize software such as ANTHEWIN, analyze the whole aminoacid sequence (GeneBank, access number: AY278488) of the gB glycoprotein of HSV1 virus by computer, screen out the strong epitope in the gB glycoprotein of HSV1 virus ( figure 1 ), that is, from the 1st amino acid to the 696th amino acid, its amino acid sequence is as follows:

[0218] Pro Ser Ser Pro Gly Thr Pro GIY Val Ala Ala Ala Thr Gln Ala Ala Asn Gly

[0219] Gly Pro Ala Thr Pro Ala Pro Pro Ala Leu GlY Ala Ala Pro Thr GlY Asp Pro

[0220] Lys Pro Lys Lys Asn Lys Lys Pro Lys Asn Pro Thr Pro Pro Arg Pro Ala Gly

[0221] Asp Asn Ala Thr Val Ala Ala Gly His Ala Thr Leu Arg Glu His Leu Arg Asp

[0222] Ile Lys Ala Glu Asn Thr Asp Ala Asn Phe Tyr Val Cys Pro Pro Pro Thr Gly

[0223] Ala Thr Val Val Gln Phe Glu Gln Pro Arg Arg Cys Pro Thr Arg Pro Glu Gly

[0224] Gln Asn Tyr Thr Glu Gly Ile Ala Val Val Phe Lys Gl...

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Abstract

The invention discloses the gene fragment of the extracellular region in the virus HSV1 gB glycoprotein of chemosynthesis, expression and application, belonging to the technical field of genetic engineering, vaccine and diagnostic reagent. Through computer analysis, the invention screens out strong epitope, from the first amino acid to the 696th amino acid, 696 amino acids in all, chooses codon favored both by eukaryote and prokaryote, synthesizes chemically a brand new gene sequence of the epitope, and utilizes the genetic engineering technology to express the gene fragment and prepare the strong epitope of the virus HSV1 gB glycoprotein. The strong epitope of the virus HSV1 gB glycoprotein expressed can be used for the vaccine, the virus HSV1 antigen and antibody detection, immunizationpreparation of mono-and poly-clonal antibodies of anti-HSV1.

Description

technical field [0001] The present invention relates to a chemically synthesized brand-new gene fragment of the extracellular region of herpes simplex virus 1 (Herpes simplex virus 1, HSV1) glycoprotein B (glycoprotein B, gB glycoprotein), and uses genetic engineering technology to prepare recombinant HSV1 virus gB glycoprotein. Through computer analysis, the gB glycoprotein extracellular region fragment of HSV1 virus containing strong antigenic epitopes is screened out, the codons favored by both eukaryotic and prokaryotic organisms are selected, and a new gene sequence is chemically synthesized, and expressed by genetic engineering technology. The protein can be used for the detection of vaccines and HSV1 virus antibodies or antigens, etc. The invention relates to the fields of genetic engineering technology, vaccines and diagnostic reagents. Background technique [0002] Herpes simplex virus (HSV) is a common pathogen that endangers human health. It is easy to cause recu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/38C12N15/70C07K14/035C07K16/28A61K48/00G01N33/53G01N33/569
Inventor 李越希吕敏潘明洁杨华凤戚菁王正茂
Owner 李越希
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