Transcription activating factor as well as coding gene and application thereof

A technology of transcription activator and coding gene, which is applied in application, genetic engineering, plant gene improvement, etc., to achieve the effect of increasing expression level and enzymatic activity

Inactive Publication Date: 2008-05-21
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no reports at home and abroad on the use of chimeric transc

Method used

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  • Transcription activating factor as well as coding gene and application thereof

Examples

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Comparison scheme
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Example Embodiment

[0032] Example 1. The acquisition and functional verification of transcription activators and their coding genes that can increase the expression levels of cellulase and xylanase.

[0033] 1. Acquisition of transcription activators and their coding genes that can increase the expression levels of cellulase and xylanase

[0034] The coding gene of the transcription activator that can increase the expression level of cellulase and xylanase of the present invention contains two DNA binding domains and one activation domain; wherein the two DNA binding domains are respectively coding for Cre I promoters And the Cre I protein DNA binding domain gene (with the 23rd to 439 nucleotides from the 5'end of GENBANK number U27356) and the DNA binding domain gene encoding the ACEI protein have the 3218th from the 5'end of GENBANK number AF190793 To 3656 nucleotides), the activation domain is encoding the activation domain of the ACEII protein. The gene has the 834th to 1682th nucleotides from t...

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Abstract

The invention discloses a transcription activator, its coding gene and application. The transcription activator can increase the expression level of cellulase and xylanase, and is a protein of (a) or (b): (a) a protein consisting of the amino acid residue sequence of sequence 1 in the sequence listing; (b) Substitution and/or deletion and/or addition of one or several amino acid residues to the amino acid residue sequence of Sequence 1 in the Sequence Listing and having the function of a transcriptional activator that can increase the expression level of cellulase and xylanase is composed of (a) Derivatized protein. The transcription activator and its coding gene of the present invention can increase the expression level of cellulase and xylanase gene.

Description

technical field [0001] The invention relates to a transcription activator, its encoding gene and application, in particular to a transcription activator capable of increasing the expression levels of cellulase and xylanase, its encoding gene and application. Background technique [0002] The cellulase produced by Trichoderma has been widely used in washing, clothing, feed, paper, food and cellulosic ethanol. Trichoderma cellulase high-producing strains are often obtained by physical and chemical mutagenesis. Trichoderma can also express a certain level of xylanase and secrete it extracellularly. [0003] In Trichoderma, the expression of cellulase and xylanase is mainly regulated at the gene transcription level. Cellulase genes are a series of genes including at least 12 genes, among them, the gene with the strongest promoter is cbh1, which is responsible for the synthesis of CBHI (cellobiohydrolase I), which can account for 50% of the extracellular secreted protein %. T...

Claims

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Application Information

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IPC IPC(8): C07K14/37C12N15/31
Inventor 苏小运董志扬
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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