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Transcription activating factor as well as coding gene and application thereof

A technology of transcription activator and coding gene, which is applied in application, genetic engineering, plant gene improvement, etc., to achieve the effect of increasing expression level and enzymatic activity

Inactive Publication Date: 2008-05-21
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports at home and abroad on the use of chimeric transcription factors to enhance the expression of Trichoderma cellulase and / or xylanase

Method used

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  • Transcription activating factor as well as coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Acquisition and functional verification of transcriptional activators and their coding genes that can increase the expression levels of cellulase and xylanase.

[0033] 1. Acquisition of transcriptional activators and their encoding genes that can increase the expression levels of cellulase and xylanase

[0034] The coding gene of the transcriptional activator that can increase the expression level of cellulase and xylanase of the present invention contains two DNA binding domains and an activation domain; wherein, the two DNA binding domains are respectively coding Cre I promoters And the DNA binding domain gene of Cre I protein (the 23rd to 439th nucleotides at the 5' end from GENBANK number U27356) and the 3218th nucleotide at the 5' end of AF190793 from the ACEI protein DNA binding domain gene to 3656 nucleotides), the activation domain is the 834th to 1682th nucleotides at the 5' end of GENBANK number AF220671 for the activation domain encoding ACEII pro...

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PUM

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Abstract

The invention discloses a transcription activator, its coding gene and application. The transcription activator can increase the expression level of cellulase and xylanase, and is a protein of (a) or (b): (a) a protein consisting of the amino acid residue sequence of sequence 1 in the sequence listing; (b) Substitution and / or deletion and / or addition of one or several amino acid residues to the amino acid residue sequence of Sequence 1 in the Sequence Listing and having the function of a transcriptional activator that can increase the expression level of cellulase and xylanase is composed of (a) Derivatized protein. The transcription activator and its coding gene of the present invention can increase the expression level of cellulase and xylanase gene.

Description

technical field [0001] The invention relates to a transcription activator, its encoding gene and application, in particular to a transcription activator capable of increasing the expression levels of cellulase and xylanase, its encoding gene and application. Background technique [0002] The cellulase produced by Trichoderma has been widely used in washing, clothing, feed, paper, food and cellulosic ethanol. Trichoderma cellulase high-producing strains are often obtained by physical and chemical mutagenesis. Trichoderma can also express a certain level of xylanase and secrete it extracellularly. [0003] In Trichoderma, the expression of cellulase and xylanase is mainly regulated at the gene transcription level. Cellulase genes are a series of genes including at least 12 genes, among them, the gene with the strongest promoter is cbh1, which is responsible for the synthesis of CBHI (cellobiohydrolase I), which can account for 50% of the extracellular secreted protein %. T...

Claims

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Application Information

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IPC IPC(8): C07K14/37C12N15/31
Inventor 苏小运董志扬
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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