Method for shortening cultivation period of Fugu flavidus
A technology of red-fin pufferfish and body weight, which is applied in the field of aquatic biological genetics and breeding, can solve the problems of shortened breeding cycle, long breeding cycle and high breeding cost, and achieves the effects of reducing breeding cost, shortening breeding period and improving breeding efficiency.
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Embodiment 1
[0024] Location: Fishery Research Institute of Hebei Province Redfin Puffer Breeding Farm
[0025] This embodiment takes the following steps:
[0026] (1) Select breeding broodstock
[0027] On March 15, 2006, 10 chrysanthemum yellow puffers and 5 red fin puffer males were selected on March 15, 2006;
[0028] (2) Maturity-promoting breeding and spawning of broodstock
[0029] Cultivate the broodstock of the chrysanthemum-yellow puffer and the red-finned puffer in separate ponds, control the water temperature at 18±1°C, change the water 1 / 3-1 / 2 every day, and feed the clamworms once at 8% of the parent fish’s weight, and promote them after 30 days. After ripening and cultivating, on April 15, the body weight of the broodstock was compared according to the injection amount: 100 mg / kg and 50 mg / kg were used to inject luteinizing releasing hormone analogue (LRH-A) to the chrysanthemum yellow puffer and red fin puffer respectively;
[0030] (3) Artificial insemination and incuba...
Embodiment 2
[0041] Location: Haiyong Farm, Changli County, Hebei Province
[0042] This embodiment takes the following steps:
[0043] (1) Select breeding broodstock
[0044] On March 10, 2006, 30 3-4 instar puffers and 15 male puffers were selected;
[0045] (2) Maturity-promoting breeding and spawning of broodstock
[0046] Cultivate the broodstock of the chrysanthemum-yellow puffer and red-finned puffer in separate ponds, control the water temperature at 18±1°C, change the water 1 / 3-1 / 2 every day, and feed the nereis once, and the feeding amount is calculated as 8% of the parent fish’s body weight , after more than 30 days of ripening cultivation, on April 20, according to the weight of the broodstock according to the injection volume: 100mg / kg and 50mg / kg, the luteinizing releasing hormone analog (LRH-A );
[0047] (3) Artificial insemination and incubation
[0048] 72 hours after the injection of oxytocin drugs, on April 23, 9 female puffer fish with mature eggs were selected fo...
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