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Use of valid target of periplaneta americana

A technology of American cockroach and effective parts, which is applied in the field of medicine

Inactive Publication Date: 2008-09-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The above research results suggest the possibility of cockroach medicinal materials as antiviral active ingredients. After investigation, it was found that there was no such thing as the main drug for the preparation of medium and large polar extracts of Periplaneta americana alcohol extracts after column chromatography. Related reports or patents on pharmaceutical preparations for preventing and treating type I and type II herpes simplex virus infection

Method used

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  • Use of valid target of periplaneta americana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: The preparation method of the alcoholic extract of Periplaneta americana enriched by polyamide

[0028] 1.1 Preparation of Periplaneta americana Alcoholic Extract Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of 55% ethanol, soak and extract at room temperature, extract 3 times, each time for 24 hours. The extracts were combined, concentrated to dryness under reduced pressure, and dried in vacuum to obtain the alcoholic extract of Periplaneta americana.

[0029] 1.2 Preparation of different polyamide extracts 20 grams of alcoholic extract of Periplaneta americana, mixed with 30 grams of polyamide (10-30 mesh) to absorb and mix the sample, put it on the column, and first eluted with water until it was colorless. Continue to use 10% ethanol-water solution to elute until colorless; then use 30% ethanol-water solution to elute to colorless, take 10 ml of eluate and concentrate it to dryness under reduced pressure, freeze and dry in vacuum t...

Embodiment 2

[0030] Example 2: Enrichment of cockroach effective parts freeze-dried powder raw material with D-101 macroporous adsorption resin

[0031] 2.1 Preparation of Periplaneta americana Alcoholic Extract Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of 55% ethanol, soak and extract at room temperature, extract 3 times, each time for 24 hours. The extracts were combined, concentrated to dryness under reduced pressure, and dried in vacuum to obtain the alcoholic extract of Periplaneta americana.

[0032] 2.2 D-101 Macroporous Adsorption Resin Column Chromatography Sectional Preparation of Different Extracts: Periplaneta americana Alcoholic Extract 20 grams, with 30 grams of D-101 Macroporous Adsorption Resin Adsorption and Mixing Samples, 200 grams of D-101 Macroporous Adsorption Resin column, first eluted with water until colorless, then eluted with 30% ethanol-water solution until colorless, took 10 ml of the eluent and concentrated it to dryness under reduce...

Embodiment 3

[0035] Embodiment 3: microcytopathic inhibition (CPE) method research cockroach extract I-60 and II-60 to the inhibitory action test of HSV-1:

[0036] 1. Cell culture and virus passage:

[0037] The Vero cells were cultured in RPMI1640 medium containing 10% inactivated calf serum, 100 U / ml penicillin and 100 μg / ml in an incubator at 37°C, 5% carbon dioxide, and 100% relative humidity. Add HSV-1 into the long-lived Vero cell bottle, place 37°C, 5% carbon dioxide, and cultivate in an incubator with 100% relative humidity, collect the culture supernatant, and store it at low temperature.

[0038] 2. Experimental method:

[0039] 2.1 The inhibitory effect of cockroach extract I-60 and II-60 on the growth of Vero cells was determined by MTT method:

[0040] (1) Test drug: cockroach extract I-60, which is the polyamide-enriched refined product of the alcoholic extract of Periplaneta americana prepared according to Example 1; cockroach extract II-60, which is prepared according to...

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Abstract

The invention provides the application of the effective fraction of a periplaneta Americana in preparing drugs for inhibiting herpesvirus (HSV) infection and preventing and treating oral ulcer and / or genital herpes. The effective fraction of the periplaneta americana is obtained by extracting fresh or dry periplaneta Americana belonging to the blattidae family with ethanol aqueous solution, purifying with macroporous adsorption resin column, and refining by eluting with ethanol solution. The effective fraction of the periplaneta Americana has distinct HSV inhibitory activity, and can be processed into medicinal preparations or health products for inhibiting HSV infection and preventing and treating related diseases or symptoms.

Description

technical field [0001] The present invention relates to the field of medical technology, in particular, to a kind of Periplaneta americana, namely the function of inhibiting herpes simplex virus of the effective part extract of cockroach, and its prevention and treatment of herpes simplex virus infection, oral ulcer and / or genital herpes medicinal use. Background technique [0002] The diameter of herpes simplex virus is about 120-150 microns, the core containing DNA is located in the middle, and outwardly consists of three concentric structures of envelope, body coat, and capsid. The surface of the capsid is composed of 162 shell particles in a 3:3: Two axisymmetric icosahedrons can survive for several months at low temperature, and will be inactivated by humid heat at 50°C and drying at 90°C for 30 minutes. Herpes simplex virus can be divided into two types: type I and type II. Type I herpes virus (HSV-1) mainly infects the skin, mucous membranes and organs above the wais...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61P31/22
Inventor 王晓雨刘光明宋丽艳杨雷香何正春巫秀美彭芳董光平朱孝慈赵昱
Owner ZHEJIANG UNIV
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